结膜下注射50μmol/Lp38信号转导通路抑制剂SB203580对角膜细胞的毒性  

Toxicological effect of 50 μmol/L p38 signal transduction pathway inhibitors SB203580 via subconjunctival injection on rat corneas

作　　者：张海峰[1] 钟彦彦[1] 黄淑馨[1] 陆晓和[1] 

机构地区：[1]南方医科大学珠江医院眼科,广东省广州市510282

出　　处：《中国组织工程研究》2012年第7期1211-1214,共4页Chinese Journal of Tissue Engineering Research

基　　金：广东省科技计划项目(2008B080703037);课题名称:双氢青蒿素抑制角膜新生血管的实验与应用研究~~

摘　　要：背景:p38信号转导通路抑制剂SB203580具有抗炎及抑制肿瘤血管生长的作用,但其刺激性和毒性成为眼表应用的障碍。目的:观察结膜下注射SB203580对大鼠角膜的毒性作用。方法:分别于SD大鼠右眼结膜下注射0.04mL50μmol/LSB203580或等量生理盐水,1次/d,共7d。注射后第7天记录各组角膜炎症指数,并摘取角膜行组织学及透射电镜检查。结果与结论:裂隙灯显微镜下观察发现SB203580注射部位结膜呈可逆性贫血状改变,在注射次日消失;注射SB203580或生理盐水后,大鼠角膜炎症指数差异无显著性意义(P>0.05);但注射生理盐水后睫状充血程度重于注射SB203580的大鼠(P<0.05);组织学检查发现所有大鼠角膜上皮层完整,基质层排列规则,内皮层连续,透射电镜观察发现细胞结构及细胞器无明显异常。提示结膜下注射0.04mL50μmol/LSB2036580对大鼠角膜无明显毒性作用。BACKGROUND：SB203580 which is one of the special inhibitors of p38 signal transduction has the anti-inflammatory ability and can inhibit the growth of tumor vessels.However,the toxicity and irritation of SB203580 may be the obstacle of the application on eyes.OBJECTIVE：To study the toxicological effect of SB203580 with subconjunctival injection on rat corneas.METHODS：Right eyes of SD rats were preformed with subconjunctival injection of 0.04 mL of 50 μmol/L SB203580 and saline in the same dose respectively,once per day for 7 days.At 7 days after injection,the corneal inflammation index was recorded and all the right corneas were harvested for histopathological and ultrastructural examination.RESULTS AND CONCLUSION：Reversible anemia-like conjunctiva was observed in SB203580 group and disappeared at the second day after injection;After injection with SB203580 and saline,no significant difference of corneal inflammation index was found between the two groups（P 0.05）,but the ciliary congestion score in saline group was higher than that in SB203580 group（P 0.05）.Histological examination revealed that all rat corneal epithelium were completed,the stroma was arranged in order and the cortex was continuous.No obvious abnormal corneal structures and organelles were found under transmission electron microscope.This suggests that subconjunctival injection of 0.04 mL of 50 μmol/L SB2036580 had no obvious toxicological effect on rat corneas.

关 键 词：角膜 SB203580 P38 毒性 抗炎 

分 类 号：R318[医药卫生—生物医学工程]