生肌象皮膏与无象皮处方生肌膏的体外抗炎及免疫调节作用  

Anti-inflammatory and immunomodulation effect of Shengji corium elephatis mastic or Shengji mastic without elephant skin in vitro

作　　者：杜韦静[1] 陈宝元[2] 薛晓东[1] 刘芳[1] 

机构地区：[1]天津中医药大学,天津市300073 [2]天津中医药大学第二附属医院中医外科,天津市300193

出　　处：《中国组织工程研究》2012年第7期1290-1294,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(30873269)~~

摘　　要：背景:出于野生动物保护要求,对含有象皮处方的中药在评价成为重要研究课题。目的:比较生肌象皮膏与无象皮处方生肌膏在抗炎及免疫调节方面的药理作用,考察象皮在处方中的作用和地位。方法:在小鼠单核/巨噬细胞株RAW264.7细胞中加入脂多糖进行诱导,然后再分别加入生肌象皮膏(0,125,250,500,1000mg/L)与无象皮处方生肌膏(0,125,250,500,1000mg/L)水提取物。结果与结论:生肌象皮膏与无象皮生肌膏水提取物在125～1000mg/L范围内对RAW264.7细胞增殖无抑制作用(P>0.05),且125mg/L生肌象皮膏水提物对RAW264.7细胞增殖活性有促进作用(P<0.05)。与单独脂多糖刺激相比,生肌象皮膏能显著抑制脂多糖诱导的RAW264.7细胞一氧化氮分泌及细胞炎性因子肿瘤坏死因子α和白细胞介素6mRNA和蛋白的表达,显示了较强的抗炎作用,无象皮生肌膏也显示了一定的抗炎作用,但两组间差异显著性略差。提示象皮并不是生肌象皮膏抗炎作用的主要药效成分,从野生动物保护的角度出发,可考虑省略象皮或由其他药材代用象皮。BACKGROUND：The revalue of the traditional Chinese medicine with the prescription of elephant skin is an important research subject for the purpose of wild animal protection.OBJECTIVE：To compare the pharmacological actions of anti-inflammatory and immunomodulation effect of Shengji corium elephatis mastic and Shengji mastic without elephant skin and to explore the role of elephant skin in the prscription.METHODS：Mouse monocyte /macrophage cell line RAW 264.7 cells were treated with lipopolysaccharide and administrated with different concentrations of water extracts from Shengji corium elephatis mastic（0,125,250,500,1 000 mg/L） and Shengji mastic without elephant skin（0,125,250,500,1 000 mg/L）.RESULTS AND CONCLUSION：Water extracts from Shengji corium elephatis mastic and Shengji mastic without elephant skin with the concentrations from 125 mg/L to 1000 mg/L had no inhibition effect on RAW264.7 cell proliferation（P 0.05）.And water extract from Shengji corium elephatis mastic with the concentration of 125 mg/L had a promoting effect on RAW264.7 proliferation（P 0.05）.Compared with single lipopolysaccharide stimulation group,Shengji corium elephatis mastic significantly inhibited the secretion of lipopolysaccharide-induced nitric oxide,mRNA and protein expression of cytokines（tumor necrosis factor αand interleukin-6） in RAW264.7 cells.It showed a strong anti-inflammatory action.Shengji mastic without elephant skin also showed certain anti-inflammatory effect,but the differences between groups was slightly less.Results suggest that elephant skin is not the major medicinal effective ingredient in the anti-inflammatory effects of Shengji corium elephatis mastic.It may be omitted or replaced by other medical materials for the purpose of wild animal protection.

关 键 词：生肌象皮膏 抗炎 单核/巨噬细胞 一氧化氮 炎性细胞因子 

分 类 号：R318[医药卫生—生物医学工程]