玻璃酸钠与膝骨关节炎患者关节液中基质细胞衍生因子1和基质金属蛋白酶3,9,13水平的相关性  

Correlation between stromal cell-derived factor-1 and matrix metalloproteinase 3,9 and 13 in the synovial fluid of knee osteoarthritis patients after injection of sodium hyaluronate

作　　者：李彦林[1] 王国梁[1] 曹斌[1] 马珂[1] 许鹏[1] 杨光[1] 高岗[1] 

机构地区：[1]昆明医学院第一附属医院骨科,云南省昆明市650032

出　　处：《中国组织工程研究》2012年第7期1323-1326,共4页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目资助(30860286);课题名称:SDF-1/CXCR4信号通路的干预及调节关节软骨退变的研究~~

摘　　要：背景:膝关节内滑液和软骨中玻璃酸钠在骨关节炎的发生发展中起重要作用,近来有研究表明基质细胞衍生因子1、基质金属蛋白酶3,9,13对骨关节炎软骨退变均存在影响。目的:探讨关节腔注射玻璃酸钠后对膝骨关节患者关节液中基质细胞衍生因子1和基质金属蛋白酶3,9,13水平的影响及两者之间的相关性。方法:20例膝骨关节炎并有关节积液患者于注射玻璃酸钠前及注射后1,2,3,4周抽取关节液,应用双抗体夹心ELISA法测定基质细胞衍生因子1,基质金属蛋白酶3,9,13水平并进行相关性分析。结果与结论:注射玻璃酸钠后关节液中基质细胞衍生因子1,基质金属蛋白酶3,9,13水平均有所下降,基质细胞衍生因子1、基质金属蛋白酶3,9组注射前与注射后1周,注射后1周与注射后2周比较、基质金属蛋白酶13组注射前与注射后1周比较,差异无显著性意义(P>0.05),其余各组两两相比差异均有显著性意义(P<0.05)。相关性分析结果显示基质细胞衍生因子1与基质金属蛋白酶3,9,13水平变化呈正相关。BACKGROUND：Sodium hyaluronate localized in the synovial fluid and cartilage of knee joint plays an important role in the occurrence and development of osteoarthritis.Recent studies have shown that stromal cell-derived factor-1,matrix metalloproteinases 3,9 and 13 affect the cartilage degeneration in osteoarthritis.OBJECTIVE：To explore the effects of intra-articular injection of sodium hyaluronate on the expression levels of stromal cell-derived factor 1 and matrix metalloproteinases 3,9 and 13 in the knee joint fluid of patients with osteoarthritisn and the correlation between them.METHODS：A total of 20 cases of knee osteoarthritis patients with synovial fluid were involved in this study.Joint fluid was extracted before the injection of sodium hyaluronate,in the 1st,2nd,3rd and 4th weeks after injection.The levels of stromal cell-derived factor 1,matrix metalloproteinases 3,9 and 13 in the synovial fluid were measured by double antibody sandwich ELISA assay.The correlation between them was analyzed.RESULTS AND CONCLUSION：After intra-articular injection of sodium hyaluronate,the level of stromal cell-derived factor 1,matrix metalloproteinases 3,9 and 13 in the synovial fluid decreased.In the groups of stromal cell-derived factor 1,matrix metalloproteinases 3 and 9,there was no significant difference before injection and in 1 week after injection,1 week after injection and 2 weeks after injection（P 0.05）.There was significant difference in other groups compared with each other（P 0.05）.The correlation between stromal cell-derived factor 1 and matrix metalloproteinases 3,9 and 13 was positive.

关 键 词：基质细胞衍生因子1 骨关节炎 玻璃酸钠 基质金属蛋白酶3 基质金属蛋白酶9 基质金属蛋白酶13 

分 类 号：R318[医药卫生—生物医学工程]