高效液相色谱法测定盐酸文拉法辛含量及有关物质  

Determination of Venlafaxine Hydrochloride and its Related Substances by HPLC

作　　者：杜宁[1] 周艳丽[2] 

机构地区：[1]江苏省食品药品检验所,南京210008 [2]江苏省人民医院,南京210029

出　　处：《北方药学》2012年第3期4-5,共2页Journal of North Pharmacy

摘　　要：目的:建立盐酸文拉法辛含量HPLC及有关物质的测定方法。方法:以C8柱,乙腈-0.3mol/L磷酸二氢铵(40∶60)为流动相,流量为1.0ml·min-1,柱温30℃。检测波长为230nm。结果:在浓度为(5.00～250.10)μg·mL-1范围内,本法线形关系良好,相关系数r=0.9997(n=8)。结论:该方法简单,准确,可以作为盐酸文拉法辛含量和有关物质的测定方法。Abstract：Objective： To develop a HPLC method of determinating of venlafaxine hydroehloride and its related substances. Methods： The determination was carried out on a Cs column. The mobile phase was acetonitrfle-0.3mol/L ammonium dihydrogen phosphate solution （40：60）with flow rate of 1.0 ml ·min^-1 and the column temperature was 30℃ o The detection wave-length was 230nm. Results： There was a good linearity over the range of （5.00-250.10）μg·mL^-1 with a coefficient of correlation （r）0.9997 （n=8）. Conclusion： The method was simple and accurate, it can be used as a method of quality control.

关 键 词：盐酸文拉法辛 含量测定 有关物质 HPLC 

分 类 号：R927.2[医药卫生—药学]