马来丝虫虫体抗原检测长爪沙鼠血清抗体  

DETECTION OF SERUM ANTIBODIES IN JIRDS AGAINST ANTIGENS OF BRUGIA MALAYI

作　　者：武烽[1] 王运章[1] 

机构地区：[1]河南医科大学寄生虫学教研室

出　　处：《中国寄生虫学与寄生虫病杂志》1990年第2期127-130,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金

摘　　要：长爪沙鼠实验感染马来丝虫后,用感染期幼虫、微丝蚴的超声粉碎片段抗原和成虫的冰冻切片抗原,进行间接荧光抗体试验,观察血清抗体动态变化。抗感染期幼虫抗体多于感染后前3Wk内出现,抗成虫及微丝蚴抗体多于感染后前5、6wk内出现。在微丝蚴出现前,腹腔微丝蚴阳性和阴性沙鼠抗体水平差别无显著性,而在微丝蚴出现后,抗体水平具有显著性差别。The indirect fluorescent antibody test (1FA) using the infective larva (L3), adult worm (A) and microfilaria (Mf) antigens was applied to detect anti-filarial antibodies in jirds (Meriones unguiculatus) infected with Brugia malayi. The antibodies against L3 appeared within 3 weeks after infection, followed by A and Mf. The serum antibody levels in the jirds with positive infection were compared with those in the jirds with negative infection. Before appearance of microfilaria, there was insignificant difference between these two groups. However, significant difference was observed after the appearance of microfilaria. The method of sonicating L3 and Mf fragment antigens used in this study is simple, and its results are easy to observe.

关 键 词：马来丝虫 长爪沙鼠 抗原 抗体 

分 类 号：R383.16[医药卫生—医学寄生虫学]