易善复与游泳运动对高脂饮食小鼠脂肪肝的预防作用比较  

Comparison of preventive effect of fat diet induced fatty liver between polyene phosphatidylcholine and moderate swimming exercise

作　　者：王智超[1] 刘歆阳[1] 朱亦淳[1] 梁勇臻[1] 虞娇[1] 俞彰[2] 

机构地区：[1]复旦大学上海医学院07级临床医学八年制 [2]复旦大学上海医学院电子显微镜中心实验室,上海200032

出　　处：《复旦学报（医学版）》2012年第2期166-171,共6页Fudan University Journal of Medical Sciences

摘　　要：目的分别观察保肝药易善复和游泳运动对于高脂饮食小鼠脂肪肝的预防作用,通过细胞超微结构和显微结构的比较,为临床脂肪肝的预防提供依据。方法高脂饮食性脂肪肝模型通过小鼠进食高脂饲料来诱导。将15只正常小鼠随机分为5组,即正常饮食对照组(N)、高脂饮食模型组(HF)、高脂饮食加药物组(HF+D)、高脂饮食加游泳组(HF+S)和正常饮食加药物组(N+D)。皮下注射易善复0.5mL(3.32mg/mL),游泳运动为小鼠每天25℃水温游泳20min。连续实验4周后,测定小鼠体重并观察肝细胞组织切片和超微结构形态变化,以体重变化、肝细胞中脂滴的大小和多少、肝细胞超微结构的变化为主要衡量指标,比较保肝药易善复和游泳运动对于脂肪肝的预防效果。结果 HF组小鼠肝脏内出现了明显的脂肪变性,胞质内出现大量脂滴和胆固醇结晶,体重明显高于对照组;HF+S组小鼠肝脏的显微结构和超微结构无显著变化,体重增加;HF+D组小鼠体重有明显下降,同时肝脏出现了相对较为明显的脂肪变性;N+D组小鼠在肝脏形态结构无明显变化的前提下,体重有明显降低。结论 4周的高脂饮食成功建立了小鼠脂肪肝模型;游泳运动和易善复对于抑制脂肪肝的形成有一定积极作用,游泳运动的保护作用优于易善复;易善复对于正常肝脏影响较小,可以用于脂肪肝的预防性治疗。Objective To investigate the protective and curative effects between hepatoprotective drug polyene phosphatidylcholine and moderate exercise on fat diet induced fatty liver in mice through cell ultrastructure and microstructure. The result can provide clinical basis for the prevention of fatty liver. Methods Fatty liver model in mice was induced by high fat diet. Fifteen normal mice are randomly divided into 5 groups, which are normal control （N）, high fat diet model group （HF）, high fat diet with hepatoprotective drug group （HF ＋ D）, high fat diet with swimming exercise group （HF ＋ S） and normal diet with hepatoprotective drug group （N ＋ D）. HD and ND groups were lavaged polyene phosphatidylcholine of 1.16 mg/d. HS group swam 20 minutes at 25℃ per day. After 4 weeks, we observed the weight of mice and the changes of ultrastructure and histopathologic slide in liver tissuesand the main measures are cell morphology in microstructure and submircostructure. Results In the HF group, the livers appeared obviously steatosis with lipid drops and cholesterol clefts in the cytoplasm, and the weights were higher. In HF ＋ S group, the microstructure and ultrastructure of liver tissues have no significantly change, and the weights were higher. In HF ＋ D group, the weights were reduced,however the livers appeared relatively obvious steatosis. In N ＋ D group, the microstructure and ultrastructure of the livers have no obvious change, and the weights were decreased. Conclusions Fatty liver model in mice was successfully established by 4 weeks of high fat diet along with fat metabolism disorders. Swimming and polyene phosphatidylcholine relatively control fat metabolism disorders as well as the formation of fatty liver. Polyene phosphatidylcholine is not harmful to the function and structure of healthy liver with normal diet, therefore it may be used as a preventive method to avoid fatty liver.

关 键 词：高脂饮食性脂肪肝 小鼠 易善复 游泳运动 

分 类 号：R459.7[医药卫生—急诊医学]