Tetramycin抗性基因克隆及生物活性的测定  

Determination of Resistance Gene Cloning and Biological Activity of Tetramycin

作　　者：陈飞[1] 吴红艳[1] 桓明辉[1] 关艳丽[1] 郭玲玲[1] 于广峰[1] 

机构地区：[1]辽宁省微生物科学研究院,辽宁朝阳122000

出　　处：《微生物学杂志》2012年第1期17-22,共6页Journal of Microbiology

基　　金：辽宁省自然科学基金(20082191)

摘　　要：为研究Tetramycin生物合成基因簇,提高产量,根据已知链霉菌抗性基因的保守核苷酸序列设计简并引物,以链霉菌11371基因组DNA为模板进行克隆Tetramycin抗性基因,并将其转化GS115中表达,以阳性克隆为指示菌,测定Tetramycin抗性基因生物活性。结果显示克隆的Tetramycin抗性基因1-2-1和2-1-1测定生物活性时并没有表现出对Tetramycin拮抗作用的提高,但为进一步研究Tetramycin生物合成基因簇、提高Tetramycin产量提供理论数据。In order to research gene cluster of terramycin biosynthesis and to increase the yield, a degenerate primer was designed according to the sequence of conservative nucleotide of known resistance gene of Streptomyces, and the resistance gene of tetramycin were cloned by taking the DNA of Streptomyces 11371 genome as a template, and transferred to GS115 for the expression, biological activity of the resistance gene of tctramycin were tested using positive clone as indicator strain. The results showed that resistance gene 1-2-1 and 2-1-1 of cloned tetramycin had not showed the increment of the antagonism of tetramycin while testing the biological activity, however, this had provided theoretic data for further study of gene cluster of tetramycin biosynthesis to increase the output of terramycin.

关 键 词：链霉菌 Tetramycin 抗性基因 

分 类 号：Q78[生物学—分子生物学]