栽培甘草的黄酮提取物对糖尿病大鼠血糖、尿糖及抗氧化作用的影响  

Effect of Flavonoids from the Cultivated Licorice on Blood Glucose, Urine Sugar and Antioxidation in Rats with Type II Diabetes Mellitus

作　　者：赵金英[1] 樊紫周[1] 赵伟鸿[1] 果嘉[1] 杨卫东[1] 李红兵[1] 王英华 李巍[3] 彭晓东[1,4] 

机构地区：[1]宁夏医科大学基础医学院,银川750004 [2]宁夏回族自治区药检所,银川750004 [3]日本东邦大学药效部 [4]宁夏回药现代化工程研究中心,银川750004

出　　处：《宁夏医科大学学报》2012年第2期110-114,共5页Journal of Ningxia Medical University

基　　金：国家中医药国际科技合作计划项目(2006DFA33680);宁夏医科大学重点孵育项目(XZ201002)

摘　　要：目的观察宁夏地区栽培甘草(Glycyrrhiza uralensis Fisch.)提取物甘草黄酮(licorice flavonoids,LF)对2型糖尿病(T2DM)模型大鼠的降糖作用及抗氧化作用的研究。方法采用高脂高糖饮食喂养后(12周)注射链脲佐菌素(streptozotocin,STZ,35mg.kg-1,ip)制备大鼠2型糖尿病模型,实施腹腔注射300mg.kg-1和100mg.kg-1剂量的LF干预40d后,测定空腹血糖、血浆糖化血红蛋白(HbA1C)、尿糖值、24h尿蛋白值和酮体值,以及血清超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)和总抗氧化能力(T-AOC)。结果 LF降低空腹血糖、HbA1C水平、尿糖值,升高血清SOD和GSH-PX水平的同时,降低血清MDA含量。LF对血浆T-AOC、24h尿蛋白值和酮体值,改善不明显。结论宁夏地区栽培甘草中甘草黄酮具有改善实验性2型糖尿病大鼠糖代谢的作用,其抗氧化活性可能与其作用有关。Objective To investigate the hypoglycemic effect of licorice flavonoids （LF） from cultivated lico- rice （Glycyrrhiza uralensis Fisch. ） on type II Diabetes Mellitus and its related mechanism in rats. Methods Rat model of type II diabetes mellitus （DM） was established with long- term high fat and sugar diet （12w） and intraperitoneal injection of low - dose streptozotocin （STZ, 35mg·kg-l ）. After implementation of the 300mg·kg-1 and 100mg·kg-1 doses of LF intragastrically adminstration over 40 days, the fasting blood glu- cose value, plasma glycated hemoglobin （HbA1 C） , urine sugar value, 24hr urine protein value and the ke- tones values, and serum superoxide dismutase （SOD）, malondialdehyde （MDA）, glutathione peroxidase （ GSH - PX） and total antioxidant capacity （ T - AOC ） were analyzed at the end of the test. Results LF could lower the fasting blood glucose value, lower HbA1C levels, and reduced urine sugar value ; LF could in- crease the serum levels of SOD and GSH - PX, while reducing serum MDA level. LF did no significantly im- prove the plasma T -AOC, 24hr urine protein value and the ketones value. Conclusion Ningxia cultivated licorice flavonoids can improve experimental II diabetes glucose metabolism and the antioxidant activity may be related to the effect.

关 键 词：甘草 黄酮 糖尿病 脲佐菌素 

分 类 号：R285.5[医药卫生—中药学]