一株新型鸭源呼肠孤病毒(TH11株)的分离与鉴定  

ISOLATION AND CHARACTERIZATION OF A NEW TYPE OF DUCK REOVIRUS

作　　者：陈宗艳[1] 朱英奇[1] 王世传 李传峰[1] 李露[1] 王玢缤[1] 付玉志[1] 高景鹏[1] 王超[1] 刘光清[1] 

机构地区：[1]中国农业科学院上海兽医研究所,上海200241 [2]安徽省全椒县畜牧兽医局,全椒239500

出　　处：《中国动物传染病学报》2012年第1期10-15,共6页Chinese Journal of Animal Infectious Diseases

基　　金：中央级公益性科研院所基本科研业务费专项(2012JB06;2012JB13);公益性农业科研专项(201003012);"863"计划(2011AA10A200)

摘　　要：本文从太湖流域某养鸭场分离到一株呼肠孤病毒(Duck reovirus,DRV)TH11株,该病毒感染的病鸭主要表现软脚和拉稀等主要症状,剖检病变以肝脏出血和脾脏坏死为主要特征。电镜鉴定结果显示,该病原具有呼肠孤病毒的典型形态学特征。动物回归试验和RT-PCR扩增结果证明,该株病毒无论是在致病性方面,还是在基因序列方面都不同于以往的番鸭呼肠孤病毒,而是一株对多品种鸭具有较高致病性和病死率的新型鸭呼肠孤病毒。A new infectious disease emerged on a ducks farm in Jiangsu Province. The sick ducks showed weakness in legs and diarrhea and pathological findings included necrotic foci in the spleens and hemorrhage in the livers. A virus was isolated from the liver and spleen of a dead duck. The virus isolate designated Duck reovirus（DRV） TH11 was demonstrated different from those previously documented in pathogenecity in ducks, genetic analysis in RT-PCR and morphology under electron microscopy. Therefore, the virus isolate DRV-TH11 represented a new type of duck reovirus.

关 键 词：鸭源呼肠孤病毒 动物回归试验 电镜 RT-PCR 

分 类 号：S858.322.659.4[农业科学—临床兽医学]