一株牛蛙源虹彩病毒的分离及鉴定  

CHARACTERIZATION OF AN IRIDOVIRUS ISOLATE FROM RANA CATESBIANA

作　　者：刘晓东[1] 杨金先[1] 龚晖[1] 朱春华[2] 林天龙[1] 

机构地区：[1]福建省农业科学院生物技术研究所,福州350003 [2]福建省农业科学院畜牧兽医研究所,福州350013

出　　处：《中国动物传染病学报》2012年第1期16-21,共6页Chinese Journal of Animal Infectious Diseases

基　　金：福建省科技创新平台项目(2009N2003);福建省农业科学院创新项目(STIF-Y02)

摘　　要：用鲤鱼表皮瘤细胞系(epithelioma papulosum cyprini cell line,EPC)从福建省某美洲牛蛙养殖场分离到一株病毒FJ049。感染病毒的EPC呈现细胞圆缩、颗粒增多、脱落等特征性病变。间接免疫荧光检测结果表明,感染FJ049的EPC细胞与虹彩病毒单克隆抗体反应并出现特异性的胞浆荧光。采用PCR对虹彩病毒主衣壳蛋白(major capsid protein,MCP)基因保守区域进行扩增,扩增出531 bp的特异性基因片段。MCP基因同源性和遗传进化分析结果表明分离株FJ049与沼泽绿牛蛙虹彩病毒RGV9808的核苷酸同源性最高,为99.8%,属于虹彩病毒科蛙病毒属。An iridovims strain F J049 was isolated on epithelioma papillosum cyprinid （EPC） cell line from a moribund American bullfrog （Rana catesbiana） on a farm in Fujian Province. The infected EPC cells developed cytopathogenic effects （CPE）, such as rounding, granulation and detachment. The monoclonal antibody against iridovirus showed positive reaction in the FJ049-infected EPC cells in indirect immunofluorescence assay and specific fluorescence appeared in the cytoplasm of infected cells. A specific 53 lbp fragment was amplified in PCR from the conserved regions of major capsid protein （MCP） gene. The phylogenetic analysis ofMCP gene showed that the FJ049 strain shared 99.8% identity with the RGV9808 strain isolated from Rana grylio stejneger, classified in genus Ranavirus, family Iridoviridae.

关 键 词：鲤鱼表皮瘤细胞系 牛蛙 虹彩病毒 分离 鉴定 

分 类 号：S858.992.659.1[农业科学—临床兽医学]