高效液相色谱法测定小儿至宝丸中橙皮苷含量  

Determination of Hesperidin in Xiaoer Zhibao Pills by HPLC

作　　者：马仑 韩慧琴 

机构地区：[1]河北省张家口市第四医院,河北张家口075061 [2]河北省张家口市药品检验所,河北张家口075061

出　　处：《中国药业》2012年第6期34-35,共2页China Pharmaceuticals

摘　　要：目的建立测定小儿至宝丸中橙皮苷含量的高效液相色谱法。方法采用Capcell pak-C18色谱柱(150 mm×4.6 mm,5μm),以甲醇-乙腈-0.1%磷酸水溶液(12∶14∶74)为流动相,流速为1.0 mL/min,检测波长为284 nm。结果橙皮苷进样量在0.02～2.2μg范围内与峰面积积分值呈良好线性关系(r=0.999 98),平均加样回收率为100.81%,RSD=0.65%(n=6)。结论所采用的方法简便、准确、灵敏、耐用性好,可用于小儿至宝丸的质量控制。Objective To establish a method for HPLC determination of hesperidin in Xiaoer Zhibao Ppills. Methods The Capcell PAK - C18 colunm （150 ran1 x 4.6 ram, 5 ptm） as the chromatographic column and methanol -acetonitrile -0. 1% phosphoric acid aqueous solu- tion（12： 14：74）as the mobile phase were adopted. The flow rate was 1.0 mL/min and the detective wave length was 284 nm. Results The linearity range was 0.02-2.2μg（r =0. 999 98）, the average recovery rate of hesperidin was 100.81%, RSD =0.65% （n = 6）. Conclusion The method is simple, accurate and sensitive with good durability, which can be used for the quality control of Xiaoer Zhibao Pills.

关 键 词：小儿至宝丸 橙皮苷 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]