中性植酸酶菌株的筛选及性质  

Screening of strain producing neutral phytase and its enzymatic characteristics

作　　者：朱启忠[1] 王文君[1] 黄梅娜[1] 马敏[1] 徐海峰[1] 

机构地区：[1]山东大学威海分校海洋学院,威海264209

出　　处：《生物加工过程》2012年第2期63-67,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家创新性基金资助项目(101042239);山东大学科技创新重点资助项目(A10012)

摘　　要：从天然土壤中筛选出产胞外中性植酸酶的细菌20余株,通过钼蓝法对菌种进行复筛,确定phy7为研究菌。通过16S rDNA测序分析方法鉴定该菌株属于绿脓假单胞菌属。结果表明:该菌株分泌中性植酸酶,其最适反应pH为7.0、最适反应温度为40℃;在37℃时以植酸钠为底物的Km为0.26 mmol/L,vmax为0.050 6 nmol/min。金属离子Zn2+、Al3+、Cu2+和Mn2+等对该酶有抑制作用,而Fe2+等则有一定的激活作用。About 20 neutral phytase-producing strains out of natural soil were screened. A new strain phy7 was isolated. It was identified as Pseudomonas aeruginosa by the phosphorus molybdenum blue method and 16S rDNA sequence analysis. The characteristics of the phytase were investigated. The new strain produced phytase activity and the optimal conditions were pH 7.0 and 40 ℃. For substrate dodeca- sodium phytate, Km was 0. 26 mmol/L and v was 0. 050 6 nmol/min at 40 ℃. The enzymatic activity was inhibited by Zn2＋ ,A13＋ , CH2＋ , and Mn2＋ , but activated by Fe2＋.

关 键 词：植酸酶 绿脓假单胞菌属 筛选 

分 类 号：S965.116[农业科学—水产养殖]