慢性乙型肝炎患者T淋巴细胞及激活亚群与基因型的分析  

Correlation between Genotype and T-lymphocyte and Activated T-lymphocyte Subsets in Chronic Hepatitis B

作　　者：章松平[1] 王莹[1] 陈公英[1] 

机构地区：[1]杭州市第六人民医院开放实验室,310014

出　　处：《医学研究杂志》2012年第3期65-67,共3页Journal of Medical Research

基　　金：杭州市科技发展计划(2005633Q22)

摘　　要：目的探讨慢性乙型肝炎(CHB)患者不同基因分型(B型和C型)外周血T淋巴细胞及激活亚群的表达情况。方法收集2008年11月～2010年12月杭州市第六人民医院175例CHB患者,另选取43名健康体检者作为对照。用流式细胞仪检测外周血T淋巴细胞亚群(CD3+、CD3+CD4+和CD3+CD8+)及激活亚群(CD3+HLA-DR+、CD4+HLA-DR+和CD8+HLA-DR+)的表达,荧光PCR法检测HBV基因型,化学发光法定量检测血清HBeAg含量。结果 B基因型HBeAg(+)组与C基因型组间CD8细胞百分比有显著性差异,C基因型患者CD8细胞高于B基因型。各组间CD3、CD4淋巴细胞及激活亚群均无统计学意义。结论 CHB患者不同基因分型与机体细胞免疫尤其是CD8细胞存在一定的关系。Objective To investigate the correlation between genotype and T - lymphocyte and activated T - lymphocyte subsets in chronic hepatitis B. Methods Totally 175 chronic hepatitis B patients and 43 healthy blood donors were enrolled in the study. The T - lymphocyte subsets（ CD3＋ ,CD3＋ CD4＋ and CD3＋ CD8＋ ） and activated T - lymphocyte subsets （ CD3＋ HLA - DR＋ , CD4＋ HLA - DR＋ and CD8＋ HLA - DR ＋ ） were detected by flow cytometry. HBV genotypes was detected by a real time fluorescence PCR and HBeAg was detected by chemilumine - scence method. Results Compared with the B genotype HBeAg（ ＋ ） group, the percentage of CD8 cells was increased in C genotype patients. While there was no difference in the percentage of CD3, CD4 cells and activated T - lymphocyte subsets with B genotype group and C genotype group. Conclusion There was certain relation between different genotype and cellular immune in chronic hepatitis B patients, especially CD8 ceils.

关 键 词：慢性乙型肝炎 淋巴细胞 HLA—DR抗原 基因型 

分 类 号：R512.62[医药卫生—内科学]