二维应变超声心动图评价干细胞移植对心肌梗死大鼠左心室局部收缩功能的影响  

Assessment of Regional Myocardial Function Following Mesenchymal Stem Cells Transportation in A Rat Model of Myocardial Infarction by 2-dimensional Strain Echocardiography

作　　者：廖书生[1] 阮琴韵[2] 林美燕[2] 鄢磊[2] 苏津自[3] 林茂海[3] 

机构地区：[1]温州医学院附属第一医院超声科,325000 [2]福建医科大学附属第一医院超声影像科,福州350004 [3]福建医科大学附属第一医院心血管内科,福州350004

出　　处：《医学研究杂志》2012年第3期75-78,共4页Journal of Medical Research

基　　金：福建医科大学教授学术发展基金资助项目(JS09008)

摘　　要：目的应用二维应变超声心动图(2-dimensional strain echocardiography,2DSE)观察心肌梗死大鼠在骨髓间充质干细胞(mesenchymal stem cells,MSCs)移植后局部心肌功能改善情况,探讨2DSE在心肌梗死大鼠干细胞移植研究中心功能评价的价值。方法 40只SD大鼠随机分为心肌梗死组(n=14)、MSCs治疗组(n=16)和对照组(n=10),心肌梗死组与MSCs组建立心肌梗死模型,对照组只进行假手术。MSCs组在梗死区心肌局部注射DAPI标记的MSCs,心肌梗死组和对照组只注射DMEM培养基。分别于术前1天和术后1周、术后4周用2DSE观察左心室乳头肌水平短轴局部室壁运动情况。术后4周用荧光显微镜观察心肌梗死区移植细胞分布,免疫组化检测移植细胞的肌钙蛋白表达情况。结果术后4周,MSCs治疗组局部室壁的径向应变和应变率高于心肌梗死组(P<0.01);MSCs组心肌梗死区可见DAPI标记阳性的细胞,该区肌钙蛋白表达阳性。结论 2DSE可以检测出心肌梗死大鼠在MSCs移植后局部心肌收缩功能的改善,可成为干细胞移植研究中可选择的敏感的无创性指标。Objective To investigate the value of 2 - dimensional strain echocardiography （2DSE） in a research of mesenchymal stem cells （MSCs） transplantation therapy in a rat model of myocardial infarction. Methods Forty Sprague - Dawley rats were divided into myocardial infarct （MI） group （n = 14）, MSCs treatment group （n = 16）, and control group（ n = 10）. Rat model of myocardial in- farction was established following ligation of the proximal left anterior descending coronary artery for MI - group and MSCs group, and con- trol group with sham -operation. The MSCs labeled by DAPI was implanted into the acute infarct site for the MSCs group. Myocardial strain and strain rate of left ventricular mid - anterior segment were detected by 2DSE pre - procedurally at baseline, 1 - week and 4 - week after procedures. Fluorescence microscope was used to identify the DAPI - labeled cells, and immunohistochemistry staining of Tro- ponin I was used to evaluate the differentiation and expression of engrafted MSCs. Results In the end of 4 weeks, when compared with MI group, increased strain parameters were found in MSCs group （P 〈 0.01 ）. The labeled cells were viable in the host myocardium and immunochemical staining revealed that the engrafted stem cells expressed Troponin I. Conclusion 2DSE may detect the improved region- al systolic function after transplantation of MSCs on a post - MI of rat model noninvasively.

关 键 词：超声心动图 二维应变 局部收缩功能 心肌梗死 骨髓间充质干细胞 

分 类 号：R542.2[医药卫生—心血管疾病]