肝脏干细胞研究及应用前景展望  

Prospect and advances in liver stem cells

作　　者：王楠[1,2] 邹伟[2] 刘鹏[1] 崔占峰 刘晶[1] 

机构地区：[1]大连医科大学附属第一医院中英再生医学应用研究中心,辽宁省大连市116011 [2]辽宁师范大学生命科学学院,辽宁省大连市116029 [3]Department of Engineering Science,University of Oxford

出　　处：《中国组织工程研究》2012年第6期1119-1124,共6页Chinese Journal of Tissue Engineering Research

基　　金：科技部国家科技合作专项"俄罗斯器官特异性干细胞定植支架及定向生长分化的关键技术引进与联合研发";国家自然科学基金"复层灌注微生物反应器结合hBMSCs/黑质DA能神经元3D共培养构建功能性多巴胺能神经网络的研究"(81071009);大连市科技局国际合作项目"中英干细胞临床应用与技术平台"(2009F11GH179);辽宁科技厅博士启动"三维灌注培养下BMSC成神经分化优化方案筛选及神经网络模型构建"(20091020)~~

摘　　要：背景:近年肝脏干细胞移植治疗终末期肝病得到人们的广泛关注,但临床应用中却面临来源有限,细胞数量不足,移植后干细胞往往弥散、流失,且分化率低,无法有效发挥其修复功能等技术问题制约。目的:总结肝脏干细胞组织工程的研究进展,并展望其应用前景。方法:应用计算机检索1999-01/2010-12 PubMed数据库相关文献,英文检索词"liver stem cell tissue engineering"分别与"three dimensional culture,biodegradable materials,biological reactor"组合,并限定文献语言种类为English。共检索到文献241篇,最终纳入符合标准的文献43篇。结果与结论:肝脏干细胞来源有限,而肝脏组织工程需要大量可靠的种子干细胞,三维培养是目前发现行之有效的体外扩增手段。在三维培养条件下,使用不同支架材料和反应器扩增效率及移植效果存在巨大差异,如何确定具有肝脏干细胞特异性的理想扩增条件、改善支架生物相容性以提高移植效率是关键技术问题。未来的研究需要进一步探讨肝脏干细胞特异性扩增生物反应器及肝脏干细胞特异性支架构建等技术难点。BACKGROUND： The treatment of end-stage liver disease with liver stem cells transplantation has raised the concern in recent years. However, the clinical application is restricted by some technical problems, such as cell shortage, cell dispersion and loss and low differentiation rate after transplation, which will be solved effectively with rapid development of liver stem cells tissue engineering technology. OBJECTIVE： To summarize the progress of liver stem cell tissue engineering and outlook the prospect application. METHODS： We searched for the related articles in PubMed published from January 1999 and December 2010. The key words were ＂liver stem cell tissue engineering, three dimensional culture, biodegradable materials, bioreactor＂ in English. Totally 241 articles were selected primarily, and finally 43 articles met the inclusive requirement. RESULTS AND CONCLUSION： Three-dimensional culture of cells is proved to be an effective means of cell proliferation in vitro, which will solve liver stem cells shortage in liver tissue engineering. There are big differences in proliferation efficiency and transplantation effect when the different scaffold materials and reactors are used. Therefore it is a crucial technical problem to establish the ideal proliferation conditions of liver stem cells, improve the biocompatibility of scaffold and promote the efficiency of transplantation. Further studies should be done to study the construction of scaffold materials and application of reactors for liver stem cells in the future.

关 键 词：肝脏干细胞 组织工程 三维培养 生物可降解材料 生物反应器 综述文献 

分 类 号：R318[医药卫生—生物医学工程]