运动及雌激素对去卵巢大鼠骨量和骨重建影响的对比研究  

Comparative study of the effect of exercise and estrogen on the bone mass and bone remodeling in ovariectomized rats

作　　者：陈柏龄[1] 杨晓曦[1] 李佛保[1] 黎艺强 徐栋梁[1] 谢登辉[3] 廖威明[1] 

机构地区：[1]广州市中山大学附属第一医院脊柱外科 [2]广州市妇女儿童医疗中心骨科 [3]广州市南方医科大学骨科研究所

出　　处：《上海医学》2012年第1期58-62,3,共5页Shanghai Medical Journal

摘　　要：目的对比分析运动及雌激素对去卵巢大鼠骨量和骨重建的影响,以探讨运动及激素在防治骨质疏松中的作用机制的异同及优缺点。方法将48只5月龄雌性Sparague Dawley(SD)大鼠随机分为基础对照(Basal)组、假手术(Sham)组、去卵巢(Ovx)组、去卵巢+雌激素(Ovx+e)组、去卵巢+运动(Ovx+run)组及去卵巢+雌激素+运动(Ovx+run+e)组。Ovx+e和Ovx+run+e组于去卵巢术后1周开始予己烯雌酚(0.025mg/kg)每日1次皮下注射,持续12周。Ovx+run和Ovx+run+e组于去卵巢术后1周开始置于大鼠专用跑笼进行运动训练,跑速6.4m/min,每日1次,持续12周。术后13周,对所有大鼠进行股骨骨密度(BMD)及骨组织计量学指标测定。结果 Ovx+e组的BMD显著高于Ovx组(P<0.05),Ovx+run组的BMD显著高于Ovx和Ovx+e组(P值均<0.05),Ovx+run+e组的BMD显著高于其他各组(P值均<0.01)。Ovx+e组的骨小梁面积百分率(%Tb.Ar)和骨小梁数量(Tb.N)显著高于Ovx组(P值均<0.05),而骨小梁分离度(Tb.Sp)显著低于Ovx组(P<0.01)。Ovx+run组的%Tb.Ar和Tb.N均显著高于Ovx组(P值均<0.05),但显著低于其余各组(P值均<0.05);Tb.Sp显著低于Ovx组(P<0.01),但显著高于其余各组(P值均<0.01)。Ovx+run+e组的%Tb.Ar和骨小梁厚度(Tb.Th)均显著高于各去卵巢组(P值均<0.05),Tb.N均显著高于Ovx、Ovx+run组(P值均<0.05),而Tb.Sp均显著低于Ovx、Ovx+run组(P值均<0.01)。Ovx+e组的周长百分率(%L.Pm)、骨小梁表面骨形成率(BFR/BS)、骨小梁总面积骨形成率(BFR/BV)、骨组织总面积骨形成率(BFR/TV)、骨小梁面积破骨细胞数(Oc.No)、骨小梁周长破骨细胞数(Oc.No/Pm)及骨矿化沉积率(MAR)均显著低于Ovx组(P值均<0.05)。Ovx+run组的%L.Pm、BFR/BV均显著低于Ovx组(P值均<0.01),而%L.Pm、BFR/BS、BFR/BV、BFR/TV、MAR、Oc.No和Oc.No/Pm均显著高于Ovx+e组(P值均<0.01)。Ovx+run+e组的%L.Pm、BFR/BS、BFR/BV、BFR/TV均显著高于Ovx+e组(P值均<0.01),而MAR、Oc.No、Oc.No/Pm均显著低于Ovx和Ovx+run组(P值�Objective To study the effect of exercise and estrogen on bone mass and bone remodeling in ovariectomized rats, and to explore the differences between exercise and estrogen in the prevention and treatment of postmenopausal osteoporosis. Methods A total of 48 female SD rats, 5 months old, were randomly divided into Basal, Sham, Ovx （ovariectomized）, Ovx ＋ e （ovariectomized and estrogen）, Ovx＋run （ovariectomized and running） and Ovx ＋ run ＋ e groups. Diethylstilbestrol （0. 025 mg· kg^-1· d^-1 ） was subcutaneously injected in Ovx＋e and Ovx＋run＋ e groups one week after ovariectomy for 12 weeks, while the rats in Ovx＋ run and Ovx＋ run＋ e groups were designed to run in a special cage everyday （6.4 m/min, 60 rain/d） for 12 weeks. The femur bone mineral density （BMD） and bone histomorphometry indexes were measured at week 13. Results The BMD in Ovx＋e group was significantly higher than that in Ovx group （P = 0. 029）. The BMD in Ovx ＋ run group wasincreased significantly when compared with groups Ovx and Ovx＋ e （P〈0.05）. The BMD in Ovx＋ run＋ e group was the highest among the 6 groups （P〈 0.01 ）. The percentage of bone trabecula area （% Tb. Ar） and the number of bone trabecula （Tb. N） in Ovx＋e group were much more than those in Ovx group （P〈0.05）, while the bone trabecula separation （Tb. Sp） in Ovx＋ e group was less than that in Ovx group （ P〈0.01 ）. The %Tb. Ar andTb. N in Ovx＋ run group were significantly higher than those in Ovx group （P〈0.05）, while significantly lower than those in the rest groups （P〈0.05）. But Tb. Sp was opposite （P〈0.01 ）. The % Tb. Ar and bone trabecula thickness （Tb. Th） in Ovx ＋ run＋ e group were significantly higher than those in the rest ovariectomized groups （ P〈0.05）, but Tb. Sp in Ovx ＋ run ＋ e group was lower than that in Ovx and Ovx ＋ run groups （ P〈0.01 ）. Thepercentage of perimeter （% L. Pm）, bone formation rates （BFR/BS, BFR/BV, BFR/TV�

关 键 词：运动 骨重建 骨组织计量 骨质疏松 大鼠 

分 类 号：R580[医药卫生—内分泌]