不同阳离子对Cyanea capillata水母触手提取物溶血活性的影响(英文)  

Effect of various cations on hemolytic activity of tentacle-only extract from jellyfish Cyanea capillata

作　　者：陆佳[1] 王倩倩[1] 张尉[2] 王蓓蕾[1] 王涛[1] 张林[1] 温小娟[1] 柳国艳[1] 赵杰[1] 肖良[1] 张黎明[1] 

机构地区：[1]第二军医大学海军医学系防化医学教研室,上海200433 [2]第二军医大学海军医学系,上海200433

出　　处：《第二军医大学学报》2012年第3期240-246,共7页Academic Journal of Second Military Medical University

基　　金：Supported by National Natural Science Foundation of China(41176126,81000098);the Natural Science Foundation of Shanghai Municipal Government(10ZR1437900)

摘　　要：目的探讨血浆对Cyanea capillata水母触手提取物(TOE)溶血活性的影响和孔道形成在其溶血机制中的作用。方法在具有相同红细胞浓度的1%稀释的全血和0.5%红细胞悬液中,检测不同浓度(100、200、400μg/ml)的TOE产生的溶血效应,并在两套体系中分别加入20、50或100 mmol/L的K+、Ca2+、Mg2+、Mn2+、Zn2+、Cu2+、Fe2+、La3+、NH4+等阳离子和离子螯合剂EDTA,观察其对TOE溶血活性的影响。结果在两套溶血检测体系中,TOE均呈现出剂量依赖性的溶血效应,TOE为200μg/ml时在红细胞悬液产生的溶血强度高于稀释的全血;Mn2+、Zn2+、La3+、Cu2+、Fe2+和EDTA均明显降低TOE溶血活性(P<0.05),其中以Zn2+的抑制作用最强,而K+、Ca2+、Mg2+、NH4+可不同程度地增强其溶血活性(P<0.05)。结论血浆对TOE的溶血作用有一定的保护作用,而孔道形成可能在TOE的溶血机制中具有重要作用。Objective To investigate the potential role of the pore-formation in the hemolytic activity of tentacle-only extract（TOE） from the jellyfish Cyanea capillata.Methods The effects of various cations,including K＋,Ca2＋,Mg2＋,Mn2＋,Zn2＋,Cu2＋,Fe2＋,La3＋and NH4＋ on the hemolytic activity of TOE were compared in two different test systems： 1% whole blood and 0.45% erythrocyte suspension with the same erythrocyte concentration.Results The hemolytic activities of TOE in both tests were inhibited by Mn2＋,Zn2＋,La3＋,Cu2＋ and Fe2＋,and were promoted to a minor extent by K＋,Ca2＋,Mg2＋ and NH4＋.The chelating agent EDTA also inhibited the hemolytic activity of TOE.Conclusion The pore-formation mechanism might play an important role in the hemolytic activity of TOE.

关 键 词：水母 霞水母 触手提取物 溶血 

分 类 号：R996.3[医药卫生—毒理学]