构建缓慢释放碱性成纤维细胞生长因子的脱细胞羊膜人工活性真皮  

Construction of artificial bioactive dermis using acellular amniotic membrane loaded with controlled-released basic fibroblast growth factor

作　　者：武日东[1] 唐诗[1] 苏爱云[1] 唐庆[1] 

机构地区：[1]中山大学附属第一医院整形修复外科,广东省广州市510080

出　　处：《中国组织工程研究》2012年第8期1425-1429,共5页Chinese Journal of Tissue Engineering Research

基　　金：广东省科技计划项目(2006B60501011);广东省科技计划项目(2008B030301336)~~

摘　　要：背景：目前人工皮肤替代品的种类较多,各有优缺点,仍然没有一种理想的产品应用于临床。目的：探讨构建一种可以缓慢释放碱性成纤维细胞生长因子的新型人工活性真皮的可行性。方法：组织块法培养幼儿包皮成纤维细胞;采用酶-去垢剂法制备人脱细胞羊膜;双相法制备碱性成纤维细胞生长因子-明胶-壳聚糖缓释微球;缓释微球黏附于脱细胞羊膜上;三四代成纤维细胞培养于负载缓释微球的脱细胞羊膜上。结果与结论：制备的脱细胞羊膜为白色半透明状薄膜有较高的孔隙率,空隙不规则,孔径大小为10-100nm,无细胞毒性;碱性成纤维细胞生长因子-明胶-壳聚糖缓释微球分散较均匀,呈球形,粒径均匀,球体表面比较光滑,载药率为20ng/g,包封率为80.5%,体外药物缓释曲线显示药物控释效果良好;成纤维细胞在支架表面爬行生长良好,层粘连蛋白表达较对照组高。表明将成纤维细胞种植于负载碱性成纤维细胞生长因子-明胶-壳聚糖缓释微球的脱细胞羊膜上,缓释微球能较好地黏附于脱细胞羊膜表面。BACKGROUND： There are many kinds of skin tissue substitutes with their own advantages and disadvantages. However, no one is the perfect to apply in clinical practice. OBJECTIVE： To discuss the possibility of constructing a new kind of artificial bioactive dermis loaded with controlled-released basic fibroblast growth factor （bFGF）. METHODS： Children foreskin fibroblasts were cultured with tissue method. Human acellular amniotic membrane was prepared by the enzyme-detergent method. Two-phase method was performed to prepare the sustained release microspheres of bFGF-gelatin-chitosan. The sustained release microspheres were adhered to the acellular amniotic membrane. The third and forth generations of fibroblasts were cultured on the acellular amniotic membrane loaded with sustained release microspheres. RESULTS AND CONCLUSION： The prepared acellular amniotic membrane was white and translucent film-like. It had a high porosity, and the pore size was irregular and varies from 10 to 100 nm. The surface of bFGF-gelatin-chitosan microspheres was spherical, in uniform size and smooth. The drug-loading rate and encapsulation efficiency of microspheres were 20 ng/g and 80.5% respectively. The fibroblasts showed a well creeping growth on the scaffolds, and the laminin was more than that of the control group. It is indicated that the fibroblasts can be cultured on the acellular amniotic membrane loaded with sustained release microspheres of bFGF-gelatin-chitosan, and the sustained release microspheres can be well adhered to the acellular amniotic membrane.

关 键 词：脱细胞羊膜 支架 药物控释系统 皮肤替代品 碱性成纤维细胞生长因子 

分 类 号：R318[医药卫生—生物医学工程]