徐州地区胆结石的溶剂萃取的FTIR研究  

Investigation of FTIR Spectra Analysis of Gallstones from Xuzhou Region by Solvent Extraction

作　　者：尹文萱[1] 王敬[1] 张楠[1] 王建怀[1] 鲍亚星[2] 孙存杰 李壮福[1] 

机构地区：[1]中国矿业大学化工学院,江苏徐州221116 [2]徐州市第一人民医院,江苏徐州221000 [3]徐州市第二人民医院,江苏徐州221000

出　　处：《光谱学与光谱分析》2012年第4期953-957,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(20943005);中央高校基本科研业务费专项资金项目(2010LKHX04)资助

摘　　要：胆石病是徐州地区的多发病之一,针对徐州地区临床术中取出的五类胆结石利用系列溶剂萃取,用傅里叶变换红外光谱(FTIR)分别表征萃取前后的成分、结构及形态变化。结果表明:所取的五类徐州地区胆结石分别为胆固醇型、难溶物分别为胆红素盐、蛋白质的胆固醇为主的混合型、棕色色素型和黑色色素型胆结石。蛋白质为胆结石的形成提供沉淀基质,对于胆结石成核具有重要意义;胆结石难溶物中胆固醇和羟基碳酸磷灰石相伴而生,羟基磷灰石与胆固醇存在相互作用,促使其结晶析出,从而加速胆结石的形成。结果可以为胆结石的临床诊断、治疗及预防提供一定的参考。The present paper is a preliminary exploration of the possible way the gallstones are formed. Five categories of gall- stones from clinical surgery in Xuzhou region were extracted by a series of solvents. Fourier transform infrared spectroscopy （FTIR） was used to characterize the structure of morphological changes between gallstone and residue by extracting. The gall- stone samples are from clinical surgeries in Xuzhou region where gallstone disease is quite common. Samples were extracted with a series of solvents, and then FTIR and other instrumental analysis were applied to characterize the composition, structure and morphological changes of the samples both before and after the extraction. The results show that the gallstone samples can be classified as 5 types： cholesterol-type, cholesterol-based hybrid type with salt, bilirubin and protein as its insoluble substances, brown pigment type and black pigment type gallstones. The results also indicate that protein plays a key role in gallstone nuclea- tion process by providing a sediment matrix for the formation of gallstones. Both cholesterol and carbonated hydroxyapatite are found in the insoluble substances of the samples. It is possible that cholesterol was accompanied by carbonated hydroxyapatite and there are interactions between them, and these interactions contribute to the crystallization process and speed up the forma- tion of gallstones. All the results above may provide useful references for the clinical diagnosis, treatment and prevention of gall- stones.

关 键 词：胆结石 溶剂萃取 FTIR 胆固醇/蛋白质 

分 类 号：O657.3[理学—分析化学]