γ-氨基丁酸在围植入期小鼠子宫的含量及其意义  

γ-Amino butyric Acid in peri-implantation mouse uterine contents and its significance

作　　者：罗文萍[1] 赵海[1] 刘真真[1] 谭冬梅[1] 谭毅[1] 

机构地区：[1]重庆医科大学实验动物中心,重庆400016

出　　处：《中国临床药理学与治疗学》2012年第2期154-158,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金面上项目(30270510;31171436)

摘　　要：目的:通过检测γ-氨基丁酸(GABA)在围植入期第1日到第8日小鼠子宫的含量;并腹腔注射GABA,探讨其在围植入期小鼠子宫中的含量变化及对子宫蜕膜化的影响。方法:比色法检测妊娠小鼠第1日到第8日子宫中GABA含量;并于妊娠第5日开始腹腔注射GABA,浓度分别为0.05、0.5、5g/kg,每日1次,至第8日时称重蜕膜鼓包及石蜡切片后行HE染色,测量蜕膜化区域面积。结果:与第1日比较,第3日中GA-BA含量上调(P<0.05),第4日时出现下降趋势,却在第5日植入位点处出现最大值(P<0.01),且第6到8日的非植入位点处GABA含量显著高于植入位点;体内实验发现,0.05g/kgGABA及5g/kg GABA可显著减少蜕膜鼓包数量,0.5g/kgGABA可显著降低蜕膜鼓包重量。同时,0.05g/kg GABA及0.5g/kg GABA可显著减少蜕膜化区域面积。结论:γ-氨基丁酸含量在围植入期小鼠子宫中呈现动态变化,并可能参与抑制妊娠小鼠子宫蜕膜化。AIM. To identify the content of GABA in peri implantation mouse uterine and its effects on decidualization by intraperitoneal injection different concentration of GABA. METHODS： The contents of GABA in peri-implantation mouse uterine from day 1 to day 8 were measured by chromatometry. From day 5, 0.05 g/kg, 0.5 g/kg, 5 g/kg concentrations of GABA were injected by abdominal cavity until day 8. Decidual tissues were weighted and then made paraffin section followed by HE staining. Areas of decidual were measured by Image J software. RESULTS： The contents of GABA in the pregnancy of D3 utrerine upregulated followed decreased in D4 utrerine but the highest content of GABA was occurred in D5 implantion sites uterine compared with that oi in D1 utrer ine. The content of GABA from D6 to D8 utrerine in iner- implantion sites were higher than im plantion sites. Both 0. 05 g/kg GABA and 5 g/kg GABA could reduced the number of deciduum, while both 0.5 g/kg GABA could down- regulated the weight of deciduum. Both 0.05 g/kg GABA and 0.5 g/kg GABA could reduce the area of decidual region. CONCLUSION. The contents of GABA showed dynamic change in periimplantation mouse uterine which may be invloved in inhibited decidualization.

关 键 词：Γ-氨基丁酸 比色法 蜕膜 

分 类 号：Q492[生物学—生理学] R915[医药卫生—微生物与生化药学]