125株耐碳青霉烯类抗菌药物鲍氏不动杆菌多位点序列分型  

Multilocus sequence typing of 125 carbapenem-resistant Acinetobacter baumanniiisolates

作　　者：徐杰[1,2,3] 郭洁[1] 王丽丽[2] 王玉飞[2] 苑锡铜[2] 袁静[2] 宋宏彬[2] 黄留玉[2] 张宁[1] 陈永德[1] 陈泽良[2] 刘世巍[1] 

机构地区：[1]中国中医研究院望京医院,北京100120 [2]军事医学科学院疾病预防控制所,北京100071 [3]吉林大学公共卫生学院,吉林长春130021

出　　处：《中华医院感染学杂志》2012年第6期1101-1103,共3页Chinese Journal of Nosocomiology

基　　金：国家自然基金项目(81071399);国家重大专项(2009ZX10004-205);国家重点基础研究发展规划(2009CB522602)

摘　　要：目的了解医院耐碳青霉烯类鲍氏不动杆菌的遗传特征。方法选取耐碳青霉烯类鲍氏不动杆菌分离株,进行多位点序列分型(MLST)。结果选取的125株耐碳青霉烯类鲍氏不动杆菌,7个看家基因中6个等位基因数为1,gpi的等位基因数为3,可变位点数为3;125株菌MLST分析,3个ST型,其中ST 75型有82株,ST92型有20株,ST138型有23株,ST75、ST92与ST138属于同一克隆群。结论医院耐碳青霉烯类鲍氏不动杆菌相对进化较慢,对多种抗菌药物耐药,应严格消毒措施及无菌操作,减少由耐碳青霉烯类鲍氏不动杆菌引起的医院感染;减少抗菌药物的使用,减缓泛耐药菌的产生。OBJECTIVE To explore the genetic characteristics of carbapenem-resistant Acinetobacter baumannii isolated from the hospital.METHODS Carbapenem-resistant A.baumannii isolates were selected for multilocus sequence typing（MLST）.RESULTS Of the 125 carbapenem-resistant A.baumannii isolates selected,the valuse of six alleles were 1 in the 7 housekeeping genes,and 3 in gpi gene alleles with 3 variable sites;The MLST analysis of carbapenem-resistant isolates showed that there were 3 types of ST,including ST75 in 82 strains,ST92 in 20 srtains,and ST138 in 23 strains.ST75,ST92,and ST138 belonged to the same clonal complex.CONCLUSION The evolution of carbapenem-resistant A.baumannii isolates,which are resistant to most types of antibiotics,is relatively low;strict adherence to disinfection measures and the aseptic operation should be encouraged to control the nosocomial infections caused by carbapenem-resistant A.baumannii isolates.Reduceing the use of the antibiotics may slow down the occurrence of resistant strains.

关 键 词：鲍氏不动杆菌 多位点序列分型 看家基因多态性 克隆群 

分 类 号：R378[医药卫生—病原生物学]