MicroScan革兰阳性复合板检测葡萄球菌属诱导型克林霉素耐药的应用  

Detection of inducible clindamycin resistance applications in Staphylococcus by microScan gram-positive bacteria composite plate

作　　者：傅石明 

机构地区：[1]浙江省遂昌县中医院检验科,浙江遂昌县323300

出　　处：《中华医院感染学杂志》2012年第6期1313-1314,共2页Chinese Journal of Nosocomiology

摘　　要：目的评价MicroScan革兰阳性复合板检测葡萄球菌属诱导型克林霉素耐药的临床应用价值。方法用MicroScan微生物鉴定药敏分析仪检测203株葡萄球菌属细菌对克林霉素和红霉素的耐药性;在做药敏鉴定的同时,用已灭菌的微量加样器吸取1μg/ml克林霉素孔55μl菌液弃去,再吸取红霉素4μg/ml孔菌液55μl于1μg/ml克林霉素孔检测诱导克林霉素耐药;每株细菌均以双纸片法D-试验作对照。结果 203株葡萄球菌属中有86株对红霉素耐药而克林霉素敏感,占42.4%;其中D-试验阳性54株,诱导型克林霉素耐药菌株的检出率为62.8%;D试验阳性菌株MicroScan革兰阳性复合板检测53株阳性,两者符合率为98.8%。结论 MicroScan革兰阳性复合板可直接检测诱导克林霉素耐药。OBJECTIVE To evaluate the clinical value of the detection of inducible clindamycin resistance in Staphylococcus by MicroScan gram-positive bacteria composite plate.METHODS MicroScan semi-automatic identification and drug susceptibility analyzer was adopted in the bacteria detection of clindamycin and erythromycin resistance of 203 Staphylococcus bacteria in our hospital;while doing the susceptibility test,a sterilized micropipette was used to extract and throw away 1μg/ml bacterium erythromycin 55ul,and to extract 4μg/ml bacterium erythromycin 55μl to detect the inducible clindamycin resistance with the remain bacterium erythromycin.D-test method was performed on each strain as a control.RESULTS Among 203,86 pairs of Staphylococcus were resistant to erythromycin and sensitive to clindamycin,accounting for 42.4%.54 pairs were D-test positive,and the detection rate of inducible clindamycin resistant strains was 62.8%.53 D-test positive strains were detected positive by MicroScan gram-positive composite panels.Both results were in line with a coincidence rate 98.8%.CONCLUSION MicroScan gram-positive composite plate can be used directly in the detection of inducible clindamycin resistance.

关 键 词：MicroScan革兰阳性复合板 葡萄球菌属 诱导型克林霉素耐药 

分 类 号：R378.11[医药卫生—病原生物学]