表达神经营养因子Neurturin的骨髓间充质干细胞的构建及体外活性检测  

Establishment of Bone Marrow Mesenchymal Stem Cells Expressing Neurotrophic Factor Neurturin and Activity Detection in Vitro

作　　者：王晚璞[1] 孙茂盛[1] 李鸿钧[1] 谢天宏[1] 严敏[1] 周艳[1] 尹娜[1] 

机构地区：[1]北京协和医学院/中国医学科学院医学生物学研究所,昆明650118

出　　处：《中国生物工程杂志》2012年第3期1-6,共6页China Biotechnology

基　　金：国家自然科学基金(30971003);云南省基础研究重点项目(2007C0012Z)资助项目

摘　　要：研究神经营养因子Neurturin(NTN)在由于神经元损伤而造成的神经退行性疾病中对神经元的保护和修复作用。利用重组腺病毒载体将NTN基因转入恒河猴骨髓间充质干细胞(rMSC),通过RT-PCR、IF及Western blot方法检测NTN的转录和表达,并采用鸡胚背根神经节体外培养实验和胚胎大鼠中脑多巴胺能神经元存活实验对NTN进行体外活性检测。结果表明NTN在rMSC中稳定表达和分泌,并具有体外生物学活性,为由于神经元损伤造成的神经退行性疾病的干细胞移植治疗奠定了一定的基础。Study the protection and repairation of damaged neurons which leads to neurodegenerative diseases,neurotrophic factor Neurturin gene was transfected into Rhesus bone marrow mesenchymal stem cells（rMSC） by recombinant adenovirus.The NTN gene could be transcribed and expressed in cytoplasm of rMSC and be secreted which was detected by RT-PCR、immunofluorescence、Western blotting,and then,the culture of Chicken embryonic dorsal root ganglion test and the culture of Fetal rats midbrain dopaminergic neurons test had indicated that the NTN protein had good biological activity in vitro.The results gave a new method for stem cell transplantation to treat the neurodegenerative diseases caused by neurons damage.

关 键 词：恒河猴骨髓间充质干细胞 重组腺病毒 神经营养因子 NEURTURIN 活性检测 

分 类 号：Q784[生物学—分子生物学]