DKPs对解淀粉芽孢杆菌Q-426抗菌活性物质基因表达量的影响  

Effects of DKPs on Gene Expression of the Antibacterial Substances in Bacillus amyloliquefaciens Q-426

作　　者：熊文[1] 杨学敏[1] 王建华[2] 权春善[1,3] 范圣第[1,3] 

机构地区：[1]大连民族学院生命科学学院,大连116600 [2]中国科学院烟台海岸带研究所,烟台264003 [3]国家民委-教育部重点实验室,大连116600

出　　处：《中国生物工程杂志》2012年第3期47-52,共6页China Biotechnology

摘　　要：目的:解淀粉芽孢杆菌Q-426在其生长过程中能够产生芬枯草菌素、依枯草菌素、枯草杆菌素等多种脂肽类抗菌物质。利用实时荧光定量PCR的方法考察细菌群体感应信号分子二酮哌嗪类化合物(diketopiperazines,DKPs)对脂肽类抗菌物质合成的调控作用。方法:当Q-426菌进入对数生长期中期,向发酵液中加入终浓度为5 mg/L的DKPs,并继续培养至48 h,并利用实时荧光定量PCR的方法进行抗菌物质mRNA表达水平的定量分析。结果:二酮哌嗪类化合物能够抑制抗菌活性物质相关基因的表达。Objective： Bacillus amyloliquefaciens Q-426 could produce varieties of antifungal lipopeptides during its stationary growth process,including bacillomycin D,fengycin A and B.Effects of diketopiperazines（DKPs） as signal molecules of quorum sensing（QS） on the biosynthesis of above antifungal compounds were studied through real time fluorescent quantitative polymerase chain reaction（Real-time Q-PCR）.Methods： DKPs at a final concentration of 5 mg/l were added to the culture broth of strain Q-426 which was incubated at 30℃ for 12 h.After continuing cultivation for 48 h,quantitative analysis of mRNA expression levels was carried out by Real-time Q-PCR.Result： DKPs could inhibit the expression of some genes related with the biosynthesis of antifungal lipopeptides.

关 键 词：解淀粉芽孢杆菌Q-426 DKPs 抗菌活性 

分 类 号：Q93[生物学—微生物学]