贡柑黑腐病病原菌的分子鉴定  

Pathogen Identification of Black Rot of Gonggan

作　　者：覃旭[1] 邓明学[1] 阳廷密[1] 谭有龙[1] 张素英[1] 门友均 唐明丽[1] 张戈壁 

机构地区：[1]广西壮族自治区柑桔研究所,桂林541004

出　　处：《中国南方果树》2012年第2期10-13,共4页South China Fruits

基　　金：广西壮族自治区直属公益性科研院所基本科研业务费专项(基本业务2010-003)资助

摘　　要：贡柑黑腐病是近年来在贡柑上发生的一种新型病害,该病害可导致嫩梢快速枯死,大量嫩叶脱落,严重影响树势和产量。本研究从病区采集发病枝叶,对贡柑黑腐病病原菌进行分离、纯化,并采用rDNA-ITS序列分析技术对其进行分子鉴定。结果表明,贡柑黑腐病病原菌为链格孢属Alternaria Nees真菌,rDNA-ITS序列与GenBank中芸薹链格孢菌A.brassicae(Berk.)Sacc.和柑桔链格孢菌A.citri Ellis et Everhart的ITS序列的同源性为99%。结合形态学特征,推断该病原菌为柑桔链格孢菌。Black rot of gonggan citrus was a new disease occurred in recent years.This disease caused rapid withering of young shoot tips,massive abscission of young leaves,resulting in reduced yield.In this study,the pathogen of this disease was isolated,and identified by analysis of the rDNA-ITS sequence.The results showed that the pathogen was an Alternaria fungus.The sequence of its rDNA-ITS shared a 99% of identity with those of Alternaria brassicae（Berk.） Sacc.and Alternaria citri Ellis ＆ Everhart deposited in GenBank.The pathogen was finally identified as A.citri based on the morphological characteristics and sequence of rDNA-ITS.

关 键 词：贡柑黑腐病 病原鉴定 rDNA-ITS序列 柑桔链格孢菌 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]