电针预处理百会穴对大鼠中枢疲劳影响的初步研究  

Preliminary research on the effect of electroacupuncture pretreatment at the Baihui acupoint of rats induced with central fatigue

作　　者：郭凯[1,2] 朱锦宇[1] 段永宏[1] 胡文东[3] 朱庆生[1] 

机构地区：[1]第四军医大学西京医院全军骨科研究所 [2]解放军93801部队医院 [3]第四军医大学航空航天医学系装备教研室

出　　处：《神经解剖学杂志》2012年第2期119-124,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(60879007)

摘　　要：目的:通过电针(electroacupuncture,EA)预处理百会穴,观察大鼠中枢疲劳(central nervous system fa-tigue,CNS fatigue)后各项指标的改变,研究电针预处理对中枢疲劳的影响。方法:成年SD大鼠分6组:空白对照组(control)、电针组(EA)、1 d疲劳对照组(1 d fatigue)、2 d疲劳对照组(2 d fatigue)、1 d疲劳电针组(EA+1 dfatigue)、2 d疲劳电针组(EA+2 d fatigue)。通过负重游泳时间和旷场试验判断大鼠疲劳程度,采用高效液相色谱(High Performance Liquid Chromatography,HPLC)电化学检测法检测大鼠海马、纹状体、下丘脑和中脑内5-羟色胺(5-hydroxytryptamine,5-HT)、多巴胺(dopamine,DA)和其代谢产物的比值,研究电针预处理对疲劳的影响。结果:建模1 d后疲劳电针组的负重游泳时间比疲劳对照组显著提高(P<0.01),旷场实验结果表明疲劳电针组的自发活动明显多于疲劳对照组。HPLC结果显示建模1 d后疲劳电针组大鼠的纹状体、中脑内5-羟吲哚乙酸(5-hydroxy-indole-acetic acid,5-HIAA)/5-HT比值和下丘脑、中脑内[3,4-二羟基苯乙酸(3,4-dihydroxyphenyl-aceticacid,DOPAC)]+高香草酸(homovanillic acid,HVA))/DA比值低于疲劳对照组(P<0.05),建模2 d后四个脑区的5-HIAA/5-HT比值和海马、下丘脑内的(DOPAC+HVA)/DA比值与对照组相比均明显降低(P<0.05)。结论:电针预刺激百会穴可以改善中枢疲劳后大鼠的体力和自主活动能力,并能够减轻中枢疲劳程度。Objective： To investigate the effect of electroacupuncture（EA） pretreatment at the Baihui acupoint of rats induced with central nervous system fatigue（CNS fatigue）.Methods： Eighty male SD rats were randomly divided into six groups：（1） control;（2） EA;（3） 1 d fatigue;（4） 2 d fatigue;（5） EA＋1 d fatigue;（6） EA＋2 d fatigue.We measured weight-loaded forced swimming time、spontaneous motor activity and the 5-hydroxyindoleacetic acid（5-HIAA）/5-hydroxytryptamine（5-HT） and [3,4-dihydroxyphenyl-acetic acid（DOPAC） ＋ homovanillic acid（HVA）]/dopamine（DA） ratios,by high performance lipuid chromatography（HPLC） electrochemical detect method,in brain of rats induced with central fatigue after electroacupuncture pretreatment at the Baihui acupoint.Results： At 1 day after CNS fatigue the weight-loaded forced swimming time of EA＋fatigue group was clearly higher than that of fatigue control group（P〈0.01） and spontaneous motor activity significantly increased in EA＋fatigue group than that of fatigue control group.5-HIAA/5-HT ratio in midbrain and striatum and（DOPAC＋HVA）/DA ratio in midbrain and hypothalamus significantly decreased in EA＋fatigue group than that of fatigue control group（P〈0.05）.At 2 day after CNS fatigue 5-HIAA/5-HT ratio in all brain regions and（DOPAC＋HVA）/DA ratio in hippocampus and hypothalamus significantly decreased in EA＋fatigue group compared with that of fatigue control group（P〈0.05）.Conclusion： The present study showed that electroacupuncture pretreatment increased weight-loaded forced swimming time and spontaneous motor activity and alle viated CNS fatigue.

关 键 词：中枢疲劳 电针 高效液相色谱 自发活动 多巴胺 5-羟色胺 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]