婴儿肝外胆道闭锁肝组织MRP2及FXR蛋白的表达及其意义  

The expression of MRP2 and FXR protein in liver tissue of infants with extrahepatic biliary atresia and their significance

作　　者：唐清[1] 王琳琳[1] 王华[2] 单庆文[1] 董淳强[1] 谢湘芝[1] 

机构地区：[1]广西医科大学第一附属医院儿二科,广西南宁530021 [2]广西医科大学第一附属医院病理科,广西南宁530021

出　　处：《重庆医学》2012年第8期735-737,F0002,共4页Chongqing medicine

基　　金：广西自然科学基金(桂科青0832028);广西科学研究与技术开发计划项目(桂科攻0816004-6)

摘　　要：目的探讨肝外胆道闭锁(EHBA)婴儿肝组织中多药耐药相关蛋白2(MRP2)、法尼醇受体(FXR)蛋白的表达及其与胆汁淤积的关系。方法将临床和病理诊断为EHBA的20例患儿肝组织作为EHBA组,肝移植手术中成人供体的肝组织10例作为对照组,比较两组患者肝功能指标;采用免疫组织化学法检测肝组织中MRP2、FXR的表达情况,并进行病理图像分析。结果 EHBA组患儿血清总胆红素、直接胆红素、丙氨酸转氨酶、天冬氨酸转氨酶及γ-谷氨酰转肽酶(γ-GT)浓度明显高于对照组(P<0.05)。对照组和EHBA组肝组织MRP2蛋白吸光度(A)值分别为0.16±0.04和0.10±0.02,对照组和EHBA组肝组织FXR A值分别为0.15±0.06和0.06±0.02,EHBA组肝组织MRP2、FXR表达水平明显低于对照组(P<0.05),MRP2A值与血清γ-GT浓度呈负相关(r=-0.415,P<0.05)。FXR与MRP2的表达呈正相关(r=0.468,P<0.05)。结论 EHBA患儿肝组织MRP2表达降低可减少胆汁流,加重胆汁淤积,MRP2的下调可能与FXR相关。Objective To explore multidrug resistance associated protein2（MRP2） and farnesoid X receptor（FXR） protein expression in liver tissue of infants with extrahepatic biliary atresia （EHBA） and their relationship with cholestasis. Methods 20 samples of liver tissue of infants with clinically and pathologically confirmed EHBA were served as EHBA group, and 10 samples of liver tissue of adult liver transplant donors as control group. Liver function indexes of patients in the two groups were compared, and immunohistochemistry was conducted to detect the protein expression of MRP2 and FXR in liver tissue. Results Serum concentration of total biliruhin, direct bilirubin, alanine aminotransferase, aspartate aminotransferase and gamma-glutamyl transpeptidase（7-GT） of infants in EHBA group were significantly higher than those in control group（P〈0.05）. The absorbance（A） values of MRP2 protein in liver tissue in control and EHBA group were 0.16±0. 04,0.10 ± 0.02, respectively, and those of FXR protein in control and EHBA group were 0.15 ± 0.06,0.06±0.02, respectively. The expression levels of MRP2 and FXR in liver tissue in EHBA group were significantly lower than those in control group（P〈0.05）. The A value of MRP2 showed negative correlation with serum concentration of 7-GT（r= -0.415, P〈0.05）, while FXR and MRP2 expression was positively correlated（r= -0.415, P〈20.05 ）. Conclu- sion The down-regulation of MRP2 expression may reduce the bile flow and increase cholestasis which was associated with FXR.

关 键 词：多药耐药相关蛋白质类 胆道闭锁 法尼醇受体 婴儿 

分 类 号：R726.5[医药卫生—儿科]