Characterization of Pigeon Paramyxoviruses (Newcastle disease virus) Isolated in Kazakhstan in 2005  

作　　者：Andrey Bogoyavlenskiy Vladimir Berezin Alexey Prilipov Eugeniy Usachev Ilya Korotetskiy Irina Zaitceva Aydyn Kydyrmanov Marat Sayatov 

机构地区：[1]Institute of Microbiology & Virology, Almaty 050010, Kazakhstan [2]D.I. Ivanovsky Institute of Virology, Russian Academy of medical sciences, Moscow 123098, Russia

出　　处：《Virologica Sinica》2012年第2期93-99,共7页中国病毒学（英文版）

基　　金：USDA-ISTC partner project(K-747p,Institute of Microbiology and Virology funding,and USDA CRIS(6612-32000-038-00D)

摘　　要：Isolates of Newcastle disease virus (NDV) from deceased wild and domestic pigeons in Kazakhstan were obtained from the Almaty region during 2005 and were genotypically analyzed by using reverse transcription polymerase chain reaction (RT-PCR) with primers specific to the viral fusion (F) protein gene. Part of the amplified F protein DNA product (nucleotide sequence 47-422) and the deduced amino acid sequences were compared phylogenetically with those from strains previously reported in other geographic regions. Phylogenetic analysis indicated that the Kazakhstanian pigeon paramyxovirus type 1 (PPMV-1) isolates belong to genotype VI or 4bii. To our knowledge, this is the first reported VI isolates that possess the sequences of 112 GKRQKR116* F117 within the F0 protein. The information is fundamental to improving the efficiency of control strategies and vaccine development for NDV.Isolates of Newcastle disease virus （NDV） from deceased wild and domestic pigeons in Kazak_hstan were obtained from the Almaty region during 2005 and were genotypically analyzed by using reverse transcription polymerase chain reaction （RT-PCR） with primers specific to the viral fusion （F） protein gene. Part of the amplified F protein DNA product （nucleotide sequence 47-422） and the deduced amino acid sequences were compared phylogenetically with those from strains previously reported in other geographic regions. Phylogenetic analysis indicated that the Kazakhstanian pigeon paramyxovirus type 1 （PPMV-1） isolates belong to genotype VI or 4bii. To our knowledge, this is the first reported VI isolates that possess the sequences of 112 GKRQKR116, F 117 within the F0 protein. The information is fundamental to improving the efficiency of control strategies and vaccine development for NDV.

关 键 词：Newcastle disease virus PARAMYXOVIRUS Phylogenetic characterization PIGEON 

分 类 号：S852.65[农业科学—基础兽医学]