HIF-1α和VEGF在子宫内膜异位症增生期内膜中的表达及意义  

Expression of HIF-1α and VEGF in endometriosis at proliferative phase

作　　者：赵洁[1,2] 薛素萍[2] 曲银娥[2] 王春红[2] 

机构地区：[1]唐山市妇幼保健院妇产科,河北唐山063000 [2]河北联合大学基础医学院

出　　处：《中国实验诊断学》2012年第3期429-431,共3页Chinese Journal of Laboratory Diagnosis

基　　金：河北省教育厅科技支撑项目(2009468);唐山市科学技术研究与发展计划项目(11150205A-10)

摘　　要：目的探讨缺氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)在子宫内膜异位症(EMs)增生期内膜组织中的表达及与血管生成的相关性。方法分别用SP法和Western blot方法检测内异症40例异位内膜、33例在位内膜及36例子宫肌瘤患者(对照组)增生期内膜组织中HIF-1α和VEGF蛋白表达,CD34标记新生血管,计算微血管密度(MVD)。结果①HIF-1α和VEGF蛋白表达均为异位内膜高于在位内膜、在位内膜高于对照组内膜,组间比较差异均有统计学意义;②异位内膜MVD高于在位内膜、而在位内膜高于对照组内膜,且任意2组之间比较差异均有统计学意义;③异位内膜组织中VEGF与HIF-1α、VEGF与MVD及HIF-1α与MVD之间均存在正相关关系,差异均有统计学意义。结论 VEGF和HIF-1α可能存在相互作用,通过促进内异症血管生成,在内异症的发生发展中发挥作用。Objective To investigate the expression of HIF-1α and VEGF in endometriosis（EMs）,so as to the relationship between HIF-1α and VEGF and angiogenesis.Methods Immunohistochemistry（IHC） S-P method was used to examine the expression of HIF-1α and VEGF proteins and assess microvessel density（MVD） in 40 ectopic endometrium and 33 eutopic endometrium and 36 normal endometrium at proliferative phase,and HIF-1α and VEGF proteins were quantified by western blot（WB）.Results ①The expression of HIF-1α and VEGF in ectopic endometrium,eutopic endometrium and normal endometrium was gradually reduced significantly;②There was significant difference of MVD between ectopic endometrium and eutopic endometrium,and between eutopic endometrium and normal endometrium respectively;③There was a significant positive correlation between VEGF and HIF-1α,VEGF and MVD,HIF-1α and MVD.Conclusion HIF-1α and VEGF may have a synergetic action to promote angiogenesis in the beginning and developing of endometriosis.

关 键 词：子宫内膜异位症 缺氧诱导因子-1Α 血管内皮生长因子 微血管密度 

分 类 号：R711.71[医药卫生—妇产科学]