氧自由基对内皮祖细胞的损伤及槲皮素的保护作用  

Effects of quercetin on proliferation of human endothelial progenitor cells

作　　者：王艳[1] 胥光热[2] 彭永权[1] 刘应才[1] 

机构地区：[1]泸州医学院附属医院心内科,四川泸州646000 [2]遂宁市中心医院心内科,四川遂宁629000

出　　处：《四川医学》2012年第3期386-388,共3页Sichuan Medical Journal

摘　　要：目的研究氧自由基对内皮祖细胞(endothelial progenitor cells,EPCs)的损伤及槲皮素(quercetin,Que)的保护作用。方法分离并诱导分化人脐血EPCs,随机分为对照组、过氧化氢(H2O2)组:加入500μmol/L的H2O2建立氧化应激损伤EPCs模型、Que干预组:先分别加入浓度为60μmol/L、90μmol/L、120μmol/L的Que预处理30min后,后加500μmol/L H2O2共同培养。培养24h及48h后使用WST-1法观察EPCs增殖情况。结果与空白对照组比较,60～90μmol/L的Que可以促进EPCs增殖,120μmol/L的Que则表现出抑制EPCs增殖的作用,与培养24h比,在48h对EPCs的抑制作用较为明显。差异均有统计学意义(P<0.05)。与空白对照组比较,H2O2组EPCs增殖能力明显降低,差异有统计学意义(P<0.01)。加入60～120μmol/L的Que处理后,损伤后EPCs的细胞增殖能力得到明显改善,且具有明显的时间和浓度依赖性,与H2O2组比较,差异有统计学意义(P<0.01)。结论氧化应激损害内皮祖细胞的增殖能力,而槲皮素有显著的改善作用。Objective To investigate the possible mechanism of the injury induced by oxygen free radical to endothelial progenitor cells（EPCs）,and the protective effects of quercetin（Que） on EPCs.Methods Separated EPCs from the human umbilical cord blood,and induced the cells to differentiate.The cells then were randomly assigned into 3 groups：the control group、H2O2 group（H2O2 500μmol/L to model oxidative stress injury on EPCs）、Que group（Que 60μmol/L,90μmol/L,120μmol/L＋H2O2 500μmol/L to culture）.WST-1 assay was used to observe the proliferation of EPCs in 24 and 48 hours＇ time in the culture process.Results Que at 60～90μmol/L could stimulate EPCs proliferation,while Que at 120μmol/L represented quite the opposite compared with the control group（P0.05）.The inhibition on EPCs was more obvious in 24 hours＇s time compared with the 24 hours＇（P0.05）.The proliferation ability of EPCs was dramatically reduced in H2O2 group compared with the control group,which had apparent statistical significance（P0.01）.Pretreating with Que at 60～120μmol/L,the proliferation ability of EPCs induced by H2O2 had been improved dramatically,and it was time-dependent and dose-dependent in the experiment range,which had obvious statistical significance compared with H2O2 group（P0.01）.Conclusion Oxidative stress impairs the proliferation ability of EPCs,while Que can improve it dramatically.

关 键 词：槲皮素 氧化应激 内皮祖细胞 增殖 

分 类 号：R285[医药卫生—中药学]