辽宁绒山羊母羔活体采卵及体外受精研究  

Study on Ovum pick-up and in vitro fertilizaton of Liaoning Cashmere goat female lambs

作　　者：豆兴堂[1,2] 宋先忱[1,2] 张兴会[2] 郭丹[2] 高月[1] 

机构地区：[1]辽宁省辽宁绒山羊原种场有限公司,辽宁辽阳111000 [2]辽宁省畜牧科学研究院,辽宁辽阳111000

出　　处：《现代畜牧兽医》2012年第3期27-30,共4页Modern Journal of Animal Husbandry and Veterinary Medicine

摘　　要：本文以辽宁绒山羊母羔为研究对象,比较了COCs皮质采集与皮质髓质采集获得COCs数及可用COCs数,研究了重复超排对获得COCs数及可用COCs数的影响,比较了TALP与SOF受精体系卵裂率,以期建立胚胎体外生产技术体系。结果表明,卵巢皮质髓质采集的COCs数、可用卵母细胞数均显著高于采集卵巢皮质获得的COC(sP<0.05);重复超排获得的COCs数量、可用COCs数量均极显著低于首次超排母羔(P<0.01);SOF受精体系卵裂率显著高于TALP(P<0.05)。This dissertation raked the LiaoningCashmere Goat female lambs as the research object. First of all, number and quality of oocytes collecled by different colle tion method were compared;Secondly, studythe effect of repeationsuperovulation onnumb and quality of oocytes collected;And then, cleavage rate of TALP IVF system and SOF was co pared, to expect Creating in vitro embryo producton system. The results were showed th the number and used number of oocytes collected byovarian cortex and medulla were both si nificant higher than that of by ovarian cortex（P〈0.05）. the number and used number of o cytes collected from repeation superovulation were both very significant lower than that of first superovulation（P〈0.01）. Cleavage rate of SOF IVF system was significant higher than that of TALP（P〈0. 05）.

关 键 词：绒山羊 活体采卵 卵母细胞 胚胎 

分 类 号：S892.6[农业科学—特种经济动物饲养]