肉苁蓉中麦角甾苷含量的测定方法  

A Method for Simlultaneous Determination of Acteoside in Herbe Cistanches

作　　者：俞青芬[1] 

机构地区：[1]青海民族大学化学系,青海西宁810007

出　　处：《河北师范大学学报（自然科学版）》2012年第2期198-201,共4页Journal of Hebei Normal University：Natural Science

基　　金：国家自然科学基金(30760195)

摘　　要：用HPLC法测定肉苁蓉中麦角甾苷的含量.采用Kromasil C18（4.6 mm×250 mm,5μm）色谱柱,以甲醇-水（V（甲醇）∶V（水）=35∶65）为流动相（水中含有0.4 mL/L磷酸）等质洗脱,流速1 mL/min,检测波长331 nm,柱温35℃.检测结果表明：麦角甾苷在0.34~2.04μg范围内呈良好线性关系,回归方程为A=192.57C-2.896（r=0.999 9）,平均回收率为98.1%,RSD为1.99%（n=5）.本方法用于测定肉苁蓉中麦角甾苷的含量,重现性良好、结果准确可靠.A HPLC method for simlultaneous determination of acteoside in herbe cistanches was established.Agilgent 1100 system and Kromasil C18（4.6 mm×250 mm,5 μm）were adopted.The mobile phase consisted of H2OCH3OH（65∶35）and the detection wavelenth was 331 nm with a PDA detector.The flow rate was 1 mL/min and the column temperature was 35 ℃.The linear ranges of acteoside was 0.34～2.04 μg,the average recovery was 98.1 %,RSD was 1.99 %（n=5）.This method is accurate and reliable.It can be used to determine the content of acteoside in herbe cistanches.

关 键 词：高效液相色谱法 肉苁蓉 麦角甾苷 

分 类 号：Q657.72[生物学—生物物理学]