氯过氧化物酶催化过氧化氢氧化水溶性偶氮染料的降解  

Degradation of aqueous solube azo dyes catalyzed by chloroperoxidase with H_2O_2 as the oxidant

作　　者：张娟[1] 蒋育澄[1,2] 

机构地区：[1]陕西师范大学化学与材料科学学院,陕西西安710062 [2]陕西师范大学大分子科学陕西省重点实验室,陕西西安710062

出　　处：《工业催化》2012年第2期70-75,共6页Industrial Catalysis

基　　金：国家自然科学基金(No21176150)资助项目;中央高校基本科研业务费专项资金(GK201001005)资助项目

摘　　要：水溶性偶氮染料的脱色降解是印染行业废水处理的技术瓶颈。研究水溶液中加入H2O2,氯过氧化物酶高效催化氧化偶氮染料(橙黄Ⅳ和皂黄)的主要发色基团的性能,探讨影响染料降解率的主要因素pH、温度、H2O2用量、氯过氧化物酶用量、染料初始浓度和反应时间等。结果表明,加入H2O2,氯过氧化物酶对橙黄Ⅳ和皂黄具有较好的脱色降解效果,橙黄Ⅳ在5 min的脱色率达91.02%,皂黄在7 min的脱色率达88.83%。通过液质联用技术测定了橙黄Ⅳ染料降解的中间产物及最终产物,并以此对降解途径进行初步推测。Decolorlzahon and degradation ot water-soluble dyes is a teclameaZ botUeneck ot waste water treatment in the dyeing industry. The properties of azo bonds of the aqueous soluble azo dyes （ orange IV and metanil yellow） effectively catalysed by chloroperoxidase（CPO） with H202 as the oxidant were studied. The influencing factors such as the pH, temperature, H202 concentration, CPO dosage, decolorization time,initial concentration of the dyes and reaction time were also optimized. The results showed that chlo- roperoxidase （CPO） exhibited good decoloration rate in the degradation of aqueous soluble azo dyes using H202 as the oxidant, and the decoloration rate of orange IV for 5 mln and metanil yellow for 7 min were 91.02% and 88.83%, respectively. The intermediates and final degradation products of orange IV with CPO-H202 oxidation were detected by LC/MS. The degradation pathway was proposed accordingly.

关 键 词：催化化学 氯过氧化物酶 橙黄Ⅳ 皂黄 降解产物 

分 类 号：O643.36[理学—物理化学] TQ426.94[理学—化学]