催化动力学光度法测定痕量普卢利沙星  

Determination of trace pmlifloxacin by catalytic kinetic discoloring spectrophotometry

作　　者：符飞燕[1] 韩永和[1] 王龙彪[1] 王克军[1] 

机构地区：[1]中国人民解放军61699部队

出　　处：《现代仪器》2012年第2期85-87,共3页Modern Instruments

摘　　要：经研究发现,在缓冲溶液NaOH-KH2PO4介质中,普卢利沙星对H2O2氧化罗丹明B褪色反应有明显的抑制作用,同时又研究该阻抑催化褪色反应的动力学条件,包括酸度、试剂的用量、温度、时间对反应的影响,以及共存离子的干扰,从而建立一种新的快速测定痕量普卢利沙星的阻抑动力学光度法。测定的线性范围为2.0～30μg/mL检出限为0.5μg/mL,回归方程为A=0.0078C+0.064。相关系数r=0996。该方法简便灵敏,准确,选择性好,用于药物中普卢利沙星的测定,获得满意的效果。A catalytic kinetic spectrophotometric method for the determination of trace Prulifloxaein was developed in this paper.This method is based on the discoloring oxidation between RhodamineB and hydrogen peroxide catalyzed by pmlifloxacin in the medium of Buffer solution NaOH-KH2PO4.The influential factors of reaction and the optimum conditions for the determination of pmlifloxacin were investigated in detail.The linear determination range is between 2.0and 30μg/mL of pmlifloxacin.A detection limit of 0.5μg/ mL is achieved.The method can be applied to the determination of pmlifloxacin in tablets with satisfactory results.

关 键 词：动力学光度法 普卢利沙星 罗丹明B 

分 类 号：O614.121[理学—无机化学]