抗CD28分子单克隆抗体的制备及其免疫学特性  

Preparation and immunologic identification of the monoclonal antibodies to human CD28

作　　者：刘晶[1] 魏文青[1] 赵满仓[1] 张艳[1] 杨梦迪[1] 

机构地区：[1]北京军区总医院中心实验科,北京100700

出　　处：《中国热带医学》2012年第2期182-184,共3页China Tropical Medicine

摘　　要：目的制备抗人CD28单克隆抗体,对其免疫学特性进行分析。方法利用多肽技术合成的CD28抗原免疫BALb/c小鼠,应用细胞杂交技术,获得抗CD28的单克隆抗体,并对其免疫学特性进行了分析。结果经过融合、亚克隆成功获得了9株抗人CD28的杂交瘤细胞株,均为IgG,4株为IgG2a,5株为IgG2b。利用竞争抑制实验分别对其中的5株进行了抗原位点测定,5株分别针对三个不同的抗原位点。结论此单抗细胞株的建立,对于深入研究CD28单抗对T淋巴细胞的作用机理有重要意义。Objective To prepare the monoclonal antibody against human CD28 and analyse its immunological characteristics. Methods The human CD28 was synthesized by the FMOC solid-phase organic chemical method,then the compound antigen was immunized the BLAB/c mice to prepare the anti-CD28 monoclonal antibody,whose immunological characteristics were analyzed. Results After cell fusion and sub cloning,9 hybridoma cell lines were obtained,all of them were IgG subtypes,4 lines belonged to IgG2a,5 lines belonged to IgG2b.Five stains were chosen for anlysis of the antigenic determinant by competitive inhibition test,the results showed that the five cell lines against three different antigenic sites.Conclusion The preparation of the CD28-McAb might provide the important foundation for further study of the mechanism of CD28-McAb acting on T lymphocyte.

关 键 词：CD28 McAb T淋巴细胞 免疫学特性 

分 类 号：R392.11[医药卫生—免疫学]