黄芩苷对海人酸致痫小鼠海马白细胞介素-1β及肿瘤坏死因子-α表达的影响  

Effects of baicalin on the expression of IL-1β and TNF-α in the hippocampus of kainate-induced epileptic mice

作　　者：欧阳龙强[1] 梁日生[2] 杨卫忠[2] 石松生[2] 王春华[2] 涂献坤[2] 王立胜[1] 

机构地区：[1]福建医科大学协和临床医学院,福建省福州市350001 [2]福建医科大学附属协和医院神经外科,福建省福州市350001

出　　处：《国际神经病学神经外科学杂志》2012年第1期16-19,共4页Journal of International Neurology and Neurosurgery

基　　金：福建省自然科学基金资助项目(2011J01175)

摘　　要：目的探讨黄芩苷对海人酸诱导的小鼠癫痫持续状态后海马组织白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)表达的影响。方法将54只ICR雄性小鼠随机分为对照组、癫痫持续状态(SE)组、黄芩苷治疗组,每组18只。采用侧脑室注入海人酸建立小鼠癫痫持续状态模型。HE染色观察黄芩苷对小鼠癫痫持续状态后海马神经细胞的形态学影响。通过RT-PCR和Western blot分别检测小鼠海马组织中IL-1βmRNA、TNF-αmRNA及IL-1β、TNF-α蛋白的表达量。结果黄芩苷明显改善了SE后小鼠海马组织的病理形态学,并且可降低IL-1β、TNF-α的表达(P<0.05)。结论黄芩苷可能通过降低癫痫鼠海马组织中IL-1β、TNF-α的表达发挥抗炎作用,从而对脑组织进行保护。Objective To study the effects of baicalin on the expression of interleukin-1β （ IL-1β） and tumor necrosis factor-α （ TNF- α） in the hippocampus of kainate-induced epileptic mice. Methods Fifty-four ICR male mice were randomly divided into three groups： control, status epilepticus （SE） and baicalin groups. To establish animal model of status epilepticus, 0.1 μg/5 μ1 kainic acids was injected into the intracerebroventricular of the mice. HE staining was used to observe the pathological changes in hippocam- pus CA1 and CA3 areas. Reverse transcription polymerase chain reaction （RT-PCR） and Western blot were used to detect the expres- sion of the mRNA and protein levels of IL-1βand TNF-α, respectively. Results Baicalin significantly relieved the hippocampal injury after SE in mice, and decreased the expression of IL-1β and TNF-α （ P 〈 0.05 ）. Conclusions Baicalin may derease the expression of IL-1β and TNF-α and thus provides a neuroprotective effect on the hippocampus after SE in mice.

关 键 词：癫痫 黄芩苷 海人酸 白细胞介素-1Β 肿瘤坏死因子-Α 小鼠 

分 类 号：R285.5[医药卫生—中药学]