用斜入射光反射差法无标记实时监测不同浓度兔IgG和山羊抗兔IgG反应的动力学过程  

Label-free and real-time detection of interactional dynamic processes of rabbit IgG with different concentrations and goat anti-rabbit IgG by oblique-incidence reflectivity difference method

作　　者：何立平[1,2] 孙悦[1,2] 戴俊[2] 王京义[2] 吕惠宾[2] 王淑芳[1] 金奎娟[2] 周岳亮[2] 杨国桢[2] 

机构地区：[1]河北大学物理科学与技术学院,保定071002 [2]中国科学院物理研究所,北京凝聚态物理国家实验室,北京100190

出　　处：《物理学报》2012年第6期119-122,共4页Acta Physica Sinica

基　　金：国家重点基础研究发展计划(973计划)(批准号:2007CB935701)资助的课题~~

摘　　要：用斜入射光反射差法无标记实时监测1.25 mg/mL,2.50 mg/mL和5.00 mg/mL三种不同浓度兔IgG和浓度为0.02 mg/mL山羊抗兔IgG反应的动态过程,同时监测和获得三种不同浓度IgG反应的动力学曲线以及相应的反应时间.实验结果表明,斜入射光反射差法不仅能无标记地判别生物分子之间是否发生反应,而且能无标记实时监测生物分子反应的动力学过程,在生物分子相互作用的研究方面具有潜在和广泛的应用前景.We successfully label-free and real-time detecte the interactional dynamic processes of rabbit IgG with different concentrations （1.25 mg/mL,2.50 mg/mL and 5.00 mg/mL）,and goat anti-rabbit IgG with a concentration of 0.02 mg/mL by the oblique-incidence reflectivity difference method（OIRD）.The dynamic curves and times of interaction,response to concentration of rabbit IgG,are obtained.The experimental results demonstrate that the OIRD method can not only can distinguish the biomolecular interactions,but also real-time detect the interactional dynamic processes of biomolecules,indicating that the OIRD method has promising and extensive applications for the investigating of biomolecular interactions.

关 键 词：斜入射光反射差法 无标记和实时监测 反应动力学过程 

分 类 号：S852.43[农业科学—基础兽医学]