1α，25一二羟维生素D3对胰岛β细胞的保护作用及其机制探讨  

let, 25（OH） 2 D3 protects pancreatic β-cell line from cytokine-induced apoptosis and impaired insulin secretion

作　　者：王安平[1] 李霞[1] 超晨[1] 黄干[1] 刘碧莲[1] 彭健[1] 周智广[1] 

机构地区：[1]中南大学湘雅二医院内分泌科中南大学糖尿病中心中南大学代谢内分泌研究所糖尿病免疫学教育部重点实验室,长沙410011

出　　处：《中华医学杂志》2012年第10期695-699,共5页National Medical Journal of China

基　　金：国家自然科学基金;（81170725,81070672）;湖南省科技厅重点项目（2010SK2007）;卫生部公益性行业科研专项基金（201002002）;湖南省自然科学基金（11JJ7005）

摘　　要：目的观察1α，25一二羟维生素D3（简称VitD3）抑制白介素1B（IL-1β）和干扰素y（IFN-y）诱导胰岛β细胞凋亡的作用及对胰岛素分泌功能的影响，探讨其可能的作用机制。方法将VitD3和IL-y／IFN-y作用于细胞后，采用四甲基偶氮唑盐比色法检测细胞的生长增殖率、赫斯特荧光染色观察细胞凋亡形态变化、磷脂结合蛋白v／碘化丙啶双染色流式检测细胞凋亡率、酶联免疫分析检测葡萄糖刺激胰岛素分泌（GSIS）。结果IL-1β／IFN-y组细胞生长增殖率为32．O％±2．7％，不同浓度VitD3（10-10、10-9、10-8mol／L）＋IL-β／IFN-1β/IFN-Y组细胞生长增殖率分别为54．0％±3．2％、59．0％±1．5％，73．0％±2．1％，与IL-β／IFN-1组比较差异均有统计学意义（均P〈0．01）；Objective To explore the protective effects and potential mechanisms of 1 or, 25 （OH）2 D3 （ VitD3 ） on pancreatic B-cells. Methods The apoptosis of NIT-1 ceils was induced by interleukin-1 B （IL-1B ） and interferon-y （IFN-y） in vitro. Then the apoptotic rate of NIT-1 cells was determined by Hoechest33342 staining and Annexin V-FITC/PI flow cytometry. The insulin secretion level of NIT-1 cells was measured by ELISA. The NIT-1 cells were treated with VitD3 at the final concentrations of 10-s mol/L or underwent transient transfection with vitamin D receptor （VDR）-SiRNA. Results After the treatment of VitD3 , the apoptotic rate of NIT-1 cells decreased to 39. 7%. There were significant differences in apoptotic rate between the VitD3 treatment and IL-1B/IFN-~/ groups （ 68.4% ） （ P 〈 0.01 ）. Similarly impaired glucose-stimulated insulin secretion （ GSIS ） of NIT-1 cells recovered （ （ 7. 34 =t： 0.21 ） ng/ml ） after the treatment of VitD3 as compared with the IL-1B/IFN-~/ group （ （ 4. 88 ＋ 0. 32 ） ng/ml , P 〈 0. 01 ） . Moreover, most of the protective effects of VitD3 on pancreatic B-cells could be blocked by the transfection of VDR-SiRNA. Conclusion VitD3 may protect pancreatic B-cells from cytokinc-induced apoptosis andimpaired insulin secretion through its conjugation with VDR.

关 键 词：胰岛Β细胞凋亡 二羟维生素D3 保护作用 四甲基偶氮唑盐比色法 胰岛素分泌功能 IL-1β IFN-1 磷脂结合蛋白 

分 类 号：R587.1[医药卫生—内分泌]