一例肝衰竭婴儿外周血红细胞超微结构改变的电镜观察  

Electron microscopic observation of the ultrastructural changes of erythrocyte in peripheral blood of an infant with liver failure

作　　者：曹水良[1] 郭祖文[1] 唐福星[1] 谌崇峰[2] 宋元宗[2] 

机构地区：[1]暨南大学电镜室,广东广州510632 [2]暨南大学附属第一医院儿科,广东广州510630

出　　处：《电子显微学报》2012年第1期60-64,共5页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金资助项目(No.81070279)

摘　　要：目的:观察肝衰竭婴儿红细胞形态的超微结构变化。方法:采集一例肝衰竭婴儿的静脉血,制作扫描电镜和透射电镜样品观察红细胞的超微结构。结果:在扫描电镜下,该婴儿正常双凹圆盘形红细胞数百分比仅为2.5%,而泪滴形、靶形、口形、网织红细胞、刺形、棘形和椭圆形等多种异常形态的红细胞却高达红细胞总数的97.5%。在透射电镜下,网织红细胞和少数成熟红细胞切面具有棒状突起,突起分枝或不分枝,甚至形成了高度曲折凹陷的迷宫图形。结论:该例肝衰竭婴儿红细胞形态从外形到内部超微结构均有显著改变,提示红细胞形态多样性可能与病情复杂和严重程度有关。Objective：To study the ultrastructural changes of erythrocytes in an infant with liver-failure.Methods： Venous blood sample was collected from an infant with liver failure.Erythrocytes were observed under scanning electron microscope（SEM） and transmission electron microscope（TEM）.Results： Under SEM,the percentage of normal biconcave erythrocytes was only 1.5%,while various abnormal erythrocytes（include elongated erythrocytes,target cells,stomatocytes,reticulocytes,acanthocytes,echinocytes and other abnormal erythrocytes） took account for a proportion of 97.5%.Under TEM,reticulocytes and few mature erythrocytes show ropalocytosis.The club-shaped cells had branched or unbranched processes,which resulted in a maze with complex folds or deep invaginations of the cell membrane surface.Conclusions： The ultrastructure of erythrocytes was significantly altered in the infant with liver failure,suggesting that the morphosis of erythrocyte might be associated with the complexity and severity of the disease.

关 键 词：肝衰竭 红细胞 网织红细胞 扫描电镜 透射电镜 

分 类 号：R725.7[医药卫生—儿科]