提高微拟球藻油脂产量的培养工艺优化  

Optimization of culture parameters to increase lipid yield from Nannochloropsis oculata

作　　者：郑洪立[1] 高振[1] 尹继龙[1] 黄和[1,2] 纪晓俊[1] 孙洪磊[3] 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京210009 [2]南京工业大学材料化学工程国家重点实验室,江苏南京210009 [3]中国石油天然气股份有限公司石油化工研究院,北京100029

出　　处：《南京工业大学学报（自然科学版）》2012年第2期95-99,共5页Journal of Nanjing Tech University(Natural Science Edition)

基　　金：国家自然科学基金重点资助项目(20936002);国家重点基础研究发展计划(973计划)资助项目(2011CB200904)

摘　　要：为了提高微拟球藻油脂产量,采用二次正交旋转组合设计方法,选取NaNO3浓度、NaH2PO4浓度、温度、CO2质量分数和光照强度5个因素,对微拟球藻自养产油脂的工艺参数进行优化。得到的最佳培养工艺条件为:NaNO3质量浓度0.38 g/L、NaH2PO4质量浓度0.35 g/L、温度27.1℃、CO2质量分数6.67%、光照强度5 454 lx,预测理论最大油脂产量为0.72 g/L。在最佳工艺条件下进行验证实验,实际油脂产量0.70 g/L,是优化前的3倍多。微拟球藻油脂以C16和C18脂肪酸为主。To increase lipid yield from Nannochloropsis oculata,five culture conditions,including NaNO3 concentration,NaH2PO4 concentration,temperature,CO2 concentration,and illumination were optimized by the method of quadratic orthogonal rotation combination experiment design.The predicted theoretical maximum lipid yield was 0.72 g/L.The optimum culture conditions of NaNO3 concentration,NaH2PO4 concentration,temperature,CO2 concentration and illumination were 0.38 g/L,0.35 g/L,27.1 ℃,6.67%,and 5 454 lx,respectively.For the verification test,a lipid yield of 0.70 g/L was achieved under the optimum conditions.It was increased by two fold more than that under the original conditions.Nannochloropsis oculata were dominated by C16 and C18 fatty acids.

关 键 词：微拟球藻 培养工艺 生物柴油 二次正交旋转组合设计 

分 类 号：Q939.97[生物学—微生物学]