Mutations in Exons of the CYP17-Ⅱ Gene Affect Sex Steroid Concentration in Male Japanese Flounder(Paralichthys olivaceus)  

作　　者：MA Ruiqin HE Feng WEN Haishen LI Jifang SHI Bao SHI Dan LIU Miao MU Weijie ZHANG Yuanqing HU Jian HAN Weiguo ZHANG Jianan WANG Qingqing YUAN Yuren LIU Qun 

机构地区：[1]Fisheries College,Ocean University of China,Qingdao 266003,P.R.China

出　　处：《Journal of Ocean University of China》2012年第1期99-104,共6页中国海洋大学学报（英文版）

基　　金：supported by the Natural Science Foun-dation of Shandong Province,China(No.ZR2009DQ011);the New Teacher Special Fund of Doctor of the Ministry of Education of China(No.20090132120006);the Postdoctoral Innovative Projects of Shandong Province,China(No.200702039)

摘　　要：As a specific gene of fish,cytochrome P450c17-Ⅱ(CYP17-Ⅱ) gene plays a key role in the growth,development and reproduction level of fish.In this study,the single-stranded conformational polymorphism(SSCP) technique was used to characterize polymorphisms within the coding region of CYP17-Ⅱ gene in a population of 75 male Japanese flounder(Paralichthys olivaceus).Three single nucleotide polymorphisms(SNPs) were identified in CYP17-Ⅱ gene of Japanese flounder.They were c.G594A(p.G188R),c.G939A and c.G1502A(p.G490D).SNP1(c.G594A),located in exon 4 of CYP17-Ⅱ gene,was significantly associated with gonadosomatic index(GSI).Individuals with genotype GG of SNP1 had significantly lower GSI(P < 0.05) than those with geno-type AA or AG.SNP2(c.G939A) located at the CpG island of CYP17-Ⅱ gene.The mutation changed the methylation of exon 6.Indi-viduals with genotype AA of SNP2 had significantly lower serum testosterone(T) level and hepatosomatic index(HSI) compared to those with genotype GG.The results suggested that SNP2 could influence the reproductive endocrine of male Japanese flounder.How-ever,the SNP3(c.G1502A) located in exon 9 did not affect the four measured reproductive traits.This study showed that CYP17-Ⅱ gene could be a potentially useful candidate gene for the research of genetic breeding and physiological aspects of Japanese flounder.As a specific gene of fish,cytochrome P450c17-Ⅱ（CYP17-Ⅱ） gene plays a key role in the growth,development and reproduction level of fish.In this study,the single-stranded conformational polymorphism（SSCP） technique was used to characterize polymorphisms within the coding region of CYP17-Ⅱ gene in a population of 75 male Japanese flounder（Paralichthys olivaceus）.Three single nucleotide polymorphisms（SNPs） were identified in CYP17-Ⅱ gene of Japanese flounder.They were c.G594A（p.G188R）,c.G939A and c.G1502A（p.G490D）.SNP1（c.G594A）,located in exon 4 of CYP17-Ⅱ gene,was significantly associated with gonadosomatic index（GSI）.Individuals with genotype GG of SNP1 had significantly lower GSI（P 〈 0.05） than those with geno-type AA or AG.SNP2（c.G939A） located at the CpG island of CYP17-Ⅱ gene.The mutation changed the methylation of exon 6.Indi-viduals with genotype AA of SNP2 had significantly lower serum testosterone（T） level and hepatosomatic index（HSI） compared to those with genotype GG.The results suggested that SNP2 could influence the reproductive endocrine of male Japanese flounder.How-ever,the SNP3（c.G1502A） located in exon 9 did not affect the four measured reproductive traits.This study showed that CYP17-Ⅱ gene could be a potentially useful candidate gene for the research of genetic breeding and physiological aspects of Japanese flounder.

关 键 词：CYP17-Ⅱ gene SNPs sex steroid Japanese flounder 

分 类 号：Q523.8[生物学—生物化学] Q754