丙氨酰-谷氨酰胺对建鲤体外培养淋巴细胞增殖的影响  

Effect of Ala-Gln on lymphocyte proliferation of Cyprinus carpio var. Jian in vitro

作　　者：王桂芹[1] 芦洪梅[1,2] 韩宇田[1] 牛小天[1] 李子平[1] 赵朝阳[3] 秦贵信[1] 

机构地区：[1]吉林农业大学动物科技学院,吉林长春130118 [2]长春通威饲料有限公司,吉林长春130456 [3]中国水产科学研究院淡水渔业研究中心,农业部淡水鱼类遗传育种和养殖生物学重点开放实验室,江苏无锡214081

出　　处：《西北农林科技大学学报（自然科学版）》2012年第3期13-17,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：中国博士后基金项目(200763016);农业部淡水鱼类遗传育种和养殖生物学重点开放实验室开放基金项目(BZ2009-01)

摘　　要：【目的】探讨丙氨酰-谷氨酰胺(Ala-Gln)对免疫抑制和应激建鲤体外培养淋巴细胞增殖的调控作用。【方法】通过对建鲤注射环磷酰胺和皮质醇来建立免疫抑制和应激模型,以注射生理盐水为对照,分离头肾、脾脏和外周血淋巴细胞进行体外培养,测定培养液中不同浓度Ala-Gln(0.0,2.0,4.0,6.0,8.0和10.0mmol/L)对淋巴细胞转化率的影响。【结果】免疫抑制模型及应激模型中,建鲤头肾、脾脏和外周血的淋巴细胞转化率均显著低于对照组(P<0.05),Ala-Gln浓度在0.0～8.0mmol/L时,可以促进免疫抑制建鲤的淋巴细胞增殖(P<0.05),在0.0～6.0mmol/L时,可以促进应激建鲤的淋巴细胞增殖(P<0.05),随着Ala-Gln浓度的进一步增大,淋巴细胞转化率不再继续增大(P>0.05)。【结论】Ala-Gln对离体培养免疫抑制和应激条件下的建鲤淋巴细胞增殖具有明显的促进作用,是有潜力的鱼用抗应激免疫增强剂。[Objective] The effect of Ala-Gln on lymphocytes proliferation of Cyprinus carpio var. Jian in vitro was investigated By models of immunosuppression and stress. [Method] Models of immunosup- pression and stress were established by injecting cyclophosphamide and cortisol,and the injection of saline was taken as control. Lymphocytes of head kidney,spleen and blood were separated and cultured in vitro in media containing different Ala-Gln（0. 0,2. 0,4. 0,6. 0,8. 0 and 10.0 mmol/L）, lymphocyte proliferation was determined in different Ala-Gln concentrations of two models. [Result] Lymphocyte transformation in head Kidney, spleen and blood of control group was higher than that of immunosuppression and stressmodels（P〈0.05）, Lymphocyte proliferation of immunosuppression was significantly promoted at the range from 0.0 to 8.0 mmol/L Ala-Gln （P〈0.05） ,Lymphocyte proliferation of stress was significantly promo- ted at the range from 0.0 to 6.0 mmol/L Ala-Gln（P〈0.05）. With the increase of Ala-Gln concentration, Lymphocyte transformation no longer continued to increae（P〉0.05）. [Conclusion] In the present study,it concluded that the immune was enhanced of Ala-Gln on lymphocyte transformation of C. carpio var. Jian under stress and immunosuppression. Ala-Gln as anti-stress immunostimulants was recommended for the culture of C. carpio vat. Jian.

关 键 词：丙氨酰-谷氨酰胺 建鲤 免疫抑制 应激 淋巴细胞 

分 类 号：S942.5[农业科学—水产养殖]