冬荷抗腐烂病的初步鉴定及其生理变化  

Initiatory resistance evaluation and physiological change of the winter lotus infected rot disease

作　　者：黄勇琴[1] 李娜[1] 孔德政[1] 

机构地区：[1]河南农业大学林学院,河南郑州450002

出　　处：《西北农林科技大学学报（自然科学版）》2012年第3期111-116,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：河南省重点科技攻关项目(072102150001);河南省杰出青年基金项目(0412001900)

摘　　要：【目的】观察感染腐烂病冬荷的病情,研究不同品种冬荷抗腐烂病的生理代谢情况。【方法】以置于人工气候室盆栽冬荷"雪里红"、"傲霜"、"冬红花"3个品种和对照碗莲品种"红霞满天"为材料,人工接种腐烂病致病菌尖孢镰刀菌,在对照品种"红霞满天"发病率达25%时记录地下茎的发病情况,并取地下茎组织,测定过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)活性和总酚(TP)含量的变化。【结果】"雪里红"和"傲霜"为低抗品种,"冬红花"为高感品种;低抗品种"雪里红"和"傲霜"的POD、PPO、PAL活性和TP含量及其最大增幅均极显著高于高感品种"冬红花";各冬荷品种POD活性呈双峰曲线变化,PPO活性和TP含量呈单峰曲线变化,PAL活性变化为"S"型曲线。【结论】冬荷各品种在抗病能力和抗病生理指标值的大小上均存在差异,但在抗病生理变化趋势上是相同的。[Objective] This experiment on disease degree and physiological indexes of winter lotus in- fected rot disease was conducted to explore the physiological and biochemical responses to the disease. [Method] With potted winter lotus cv. ＂Xuelihong＂, ＂Aoshuang＂, ＂Donghongbua＂ and the control ＂Hongxiamantian＂ in the phytotron as test material,the artificial inoculation of fusarium was proceeded. After twenty-five percent inflection of the control, the subterranean stems were sampled to determine the activities of peroxidase, polyphenol oxidase,phenylalanine ammonia lyase and the content of total phenolic. [Result] The disease investigation result of winter lotus cultivars indicated that ＂Xuelihong＂ and ＂Aoshuang＂ had low resistance to the infection, and ＂Donghonghua＂ was highly sensitive; for all the en- zyme activity,the content of total phenolic and their maximum extent of augment,the low resistance ＂Xue- lihong＂ and ＂Aoshuang＂ were significantly higher than the highly sensitive ＂Donghonghua＂;among all the cultivars of winter lotus, the activity of peroxidase showed a bimodal curve, the varieation of polyphenol ox- idase activity and total phenolic content was of unimodal curve, and the activity of phenylalanine ammonia lyase showed a Sigmoid curve. [Conclusion] Among winter lotus cultivars,the disease resistance ability and physiological index value were different,but the variation trend was the same.

关 键 词：冬荷 腐烂病 抗性鉴定 生理指标 

分 类 号：S682.32[农业科学—观赏园艺] S436.461[农业科学—园艺学]