FOXP3免疫调节基因表达与异基因造血干细胞移植后急性移植物抗宿主病的关系  

Relationship between FOXP3 regulatory gene expression and graft-versus-host disease after allo-HSCT

作　　者：王立志[1,2] 秦雪梅[3] 孙元欣[3] 刘传方[3] 刘希民[2] 周芳[2] 

机构地区：[1]辽宁医学院济南军区总医院研究生培养基地,济南250000 [2]济南军区总医院 [3]山东大学齐鲁医院

出　　处：《山东医药》2012年第9期58-60,共3页Shandong Medical Journal

基　　金：山东大学自主创新基金自然科学类专项课题(2009TS065);济南市留学人员创业计划(20100407)

摘　　要：目的探讨异基因造血干细胞移植(allo-HSCT)患者移植前后外周血FOXP3免疫调节基因表达与急性移植物抗宿主病(aGVHD)的关系。方法选择21例行allo-HSCT患者,根据移植后aGVHD发生情况分为aGVHD阳性组11例、aGVHD阴性组10例。应用实时定量PCR技术检测allo-HSCT移植前、移植中及移植后外周血单个核细胞FOXP3 mRNA的表达。结果 aGVHD阳性组FOXP3 mRNA表达量较aGVHD阴性组明显降低(P<0.01),且aGVHD发生时FOXP3 mRNA表达与aGVHD发生前1～2周明显下降(P<0.05)。结论 allo-HSCT后监测患者外周血FOXP3 mRNA表达的变化可预测aGVHD发生的可能性。Objective To observe the relationship between FOXP3 regulatory gene expression in peripheral blood and acute graft-versus-host disease （aGVHD）after allo-geneic hematopoietic stem cell transplantation（allo-HSCT）. Methods The expression of FOXP3 mRNA in peripheral blood mononuclear ceils （PBMCs） of allo-HSCT patients with real-time PCR assays was detected. Results After the transplantation, the expression of FOXP3 mRNA was significantly reduced in pa- tients with aGVHD, compared with patients who did not develop clinical aGVHD （ P 〈 O. 01 ）, which had the negatively correlated with the severity of aGVHD. Paired analysis in aGVHD patients comparing the FOXP3 mRNA expression at aGVHD onset to prior time points （7-14 days） showed significantly decreased. Conclusions The expression of FOXP3 mRNA in PBMCs might be one of the useful indicators in predicting the occurrence of aGVHD.

关 键 词：血液肿瘤 造血干细胞移植 移植物排斥 FOXP3 mRNA 

分 类 号：R392.4[医药卫生—免疫学]