南海海域常见龙虾的遗传多样性分析  

Genetic Diversity of Common Spiny Lobsters in South China Sea

作　　者：刘丽[1] 杨新龙[1] 刘楚吾[1] 罗丽沙[1] 

机构地区：[1]广东海洋大学水产学院,南海水产经济动物增养殖广东省普通高校重点实验室,广东湛江524025

出　　处：《水产科学》2012年第3期160-164,共5页Fisheries Science

基　　金：国家科技支撑计划项目(2007BAD29B03);广东省科技计划项目(2010B020308004);湛江市科技攻关项目(2007C09006)

摘　　要：运用RAPD技术分析了南海海域黄斑龙虾、锦绣龙虾、密毛龙虾、杂色龙虾、波纹龙虾、中国龙虾、日本龙虾7种常见龙虾的种内和种间遗传多样性、种间亲缘关系及种质特异性标记。研究结果显示,7种龙虾的遗传多样性均较丰富,种质资源良好,其平均多态性位点比率为73.39%～89.12%,平均遗传距离为0.2134～0.4044,平均Nei基因多样性指数为0.0834～0.1990;对7个种的种间遗传距离进行聚类分析表明,日本龙虾与密毛龙虾的亲缘关系最近,与杂色龙虾的亲缘关系最远。The Random Amplified Polymorphic DNA（RAPD） was applied to detect genetic diversity,relationship and special genetic markers of common spiny lobsters Panulirus polyphagus,P.ornatus,P.penicillatus,P.versicolor,P.homarus,P.stimpsoni and P.japonicus.The percentage of polymorphic loci（P） was found to arrange from 73.39% to 89.12%,genetic distanc（D） from 0.2134 to 0.4044,and Nei＇s gene diversity（H） from 0.0834 to 0.1990.It was found that all 7 species of spiny lobsters had a relatively high genetic diversity and good germplasm resources.The dendograms based on NJ showed that P.japonicus had closer genetic relationship with P.penicillatus and further genetic relationship with P.versicolor.

关 键 词：龙虾 遗传多样性 RAPD 亲缘关系 

分 类 号：Q781[生物学—分子生物学]