一种鸡腿菇病害病原菌的分离及鉴定  

Isolation and Identification of One Kind of Pathogen from Coprinus comatus

作　　者：兰玉菲[1] 安秀荣[1] 王庆武[1] 常文程[2] 唐丽娜[1] 

机构地区：[1]泰安市农业科学研究院食用菌研究所,山东泰安271000 [2]山东农业大学植物保护学院植物病毒研究室,山东泰安271018

出　　处：《山东农业科学》2012年第3期84-87,共4页Shandong Agricultural Sciences

基　　金：国家现代农业(食用菌)产业体系建设专项(CARS-24);山东省中青年科学家科研专项基金(BS2011NY021);泰安市科技发展计划项目(20113008)

摘　　要：从莒县鸡腿菇种植区采集到表现鸡爪症状的样品,分离得到病原菌分离物RZ,采用改良的CTAB法提取其基因组总DNA,利用真菌通用引物ITS1/ITS4扩增其rDNA ITS区域序列,扩增产物纯化克隆后转化大肠杆菌DH5α,挑选阳性克隆进行测序。将获得的序列进行同源性比对和构建系统进化树,发现RZ分离物与GenBank中Bionectria ochroleuca 2个分离物聚为一簇,ITS的核苷酸一致率均为99.17%,从分子水平上证明该分离物为淡色生赤壳菌(B.ochroleuca)。The Coprinus comatus samples with the symptom of chicken claw were collected from Juxian areas, and a fungi strain named RZ was isolated. Its genome total DNA was obtained by improved CTAB meth- od. The internal transcribed spacers （ITS） were amplified by PCR using fungi universal primers ITS1 and ITS4, and the amplified products were transformed into Escherichia coli DI-ISct after purified. Then the positive clones were screened and sequenced. The homology analysis showed that RZ was clustered with two Bionectria ochroleuca isolates in GenBank and shared 99.17% of nucleotide identity in ITS, which confirmed it to be Bionectria ochroleuca.

关 键 词：鸡腿菇 病害 转录间隔区 分子鉴定 

分 类 号：S436.461[农业科学—农业昆虫与害虫防治]