不同脱水速率对“勐海大叶茶”种子脱水敏感性与抗氧化酶活性的影响  

Effect of Different Drying Rates on the Desiccation Tolerance and Antioxidant Enzyme Activities of Camellia sinensis var. assamica cv.Menghai Dayecha Seeds

作　　者：陈志欣[1,2] 包云秀[3] 郑丽[1] 兰芹英[2] 谭运洪[2] 

机构地区：[1]云南农业大学园林园艺学院,云南昆明650201 [2]中国科学院西双版纳热带植物园,云南勐腊666303 [3]云南省农业科学院茶叶研究所,云南勐海666200

出　　处：《云南农业大学学报（自然科学版）》2012年第2期241-247,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：教育部科学技术研究重点项目(208131)

摘　　要：以大叶茶品种"勐海大叶茶"(Camellia sinensis var.assamica cv.Menghai Dayecha)种子为材料,研究了脱水速率对种子的脱水敏感性与抗氧化酶活性的影响。快速脱水采用活化硅胶进行脱水处理,慢速脱水采用饱和氯化钠溶液进行脱水。结果表明:"勐海大叶茶"种子对脱水敏感,种子含水量高于44%时,慢速脱水条件下,种子的脱水耐性高于快速脱水;种子含水量低于38%时,快速脱水条件下,种子脱水耐性高于慢速脱水。脱水过程中种子细胞膜系统受到了伤害,相对电导率和TBARs含量均呈上升趋势,SOD的活性总体上呈上升的趋势;CAT呈下降的趋势;慢速脱水过程中,GR和DHAR活性被激发,在一定程度上减少了脱水伤害。The effects of different drying rates on desiccation tolerance and antioxidant enzyme activities of ＂Menghai Dayecha＂（Camelli sinensis var.assamica cv.Menghai Dayecha） seeds were studied.Rapid-dehydrated method was carried out by activated silica gel;slow-dehydrated method was carried out by a saturated solution of sodium chloride.Results showed that： ＂Menghai Dayecha＂ seeds were sensitive to dehydration,while dehydration continued seed germination rates declined.When water content was higher than 44%,seeds of ＂Menghai Dayecha＂ showed higher tolerance at slow-dehydrated method,though when water content was lower than 38%,seeds of ＂Menghai Dayecha＂ showed higher tolerance at rapid-dehydrated method.Cell membrane cleavage occurred during dehydration,as a result of relative electrolyte leakage and TBARs content ascent.Superoxide dismutase（SOD） increased during dehydration;catalase（CAT） decreased with the declining water content;glutathione reductase（GR） and dehydroascorbate reductase（DHAR） activities were inspired at slow-dehydrated method,which reducing the desiccation damage.

关 键 词：“勐海大叶茶”种子 脱水速率 抗氧化酶 膜质过氧化 脱水敏感性 

分 类 号：S565.4[农业科学—作物学]