芍甘多苷对四氯化碳亚急性肝损伤大鼠肝脏基因表达谱的影响  

Influences of effective components of paeornia and glycyrrhiza decoction （Shao Gan Duo Gan ） on liver gene expression profile in rats with subacute liver injury induced by carbon tetrachloride

作　　者：宋军[1] 赵军宁[1] 王晓东[1] 戴瑛[1] 邓治文[1] 易进海[1] 

机构地区：[1]四川省中医药科学院药理毒理研究所国家中医药管理局中药质量生物学评价重点研究室中药品质评价与中药四川省重点实验室,成都610041

出　　处：《中华生物医学工程杂志》2011年第6期487-490,共4页Chinese Journal of Biomedical Engineering

基　　金：国家十五科技攻关计划项目（2001BA701A07-22）;四川省重大科技招标项目（01SG003-33）

摘　　要：目的 研究芍药甘草汤提取物芍甘多苷对四氯化碳（CC14）亚急性肝损伤大鼠血清转氨酶、肝脏病理及肝脏基因表达谱的影响.方法 60只SD大鼠完全随机分6组（n=10）,即正常组、模型组、阳性对照组（联苯双酯100 mg/kg）和芍甘多苷低、中、高3个剂量组（88、264、528 mg/kg）.采用CCl4诱发大鼠亚急性肝损伤,然后给予不同剂量的芍甘多苷灌胃,观察药物对血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、肝脏组织病理的影响；并运用基因表达谱芯片技术,研究其对肝脏基因表达谱的影响.结果 与模型组比较,芍甘多苷高、中剂量灌胃给药均能显著降低CCl4亚急性肝损伤大鼠血清ALT[ （218.37± 114.59,234.59± 109.33） U/L比（389.12±142.57） U/L,P＜0.05]；且高剂量芍甘多苷可以显著降低肝脏组织学评分（2.42±0.52比3.92±0.84,P＜0.05）.芍甘多苷能上调和（或）下调与肝损伤相关的多种基因的表达.结论 芍甘多苷有明显的肝脏保护作用,其机制与调节与肝损伤相关的多种基因的表达有关,是多靶点作用药物.Objective To investigate influences of effective components of paeornia and glycyrrhiza decoction （Shao Gan Duo Gan） on serum transaminase,liver pathology and liver gene expression profile in rats with subacute liver injury induced by carbon tetrachloride.Methods Sixty SD rats were randomized into 6 groups：normal,model,positive control （biphenyl dimethylester 100 mg/kg）,and 3 Shao Gan Duo Gan treatment groups （with doses of 88,264,528 mg/kg）.Subacute liver injury was induced by carbon tetrachloride in the rats which were then treated by intragastric administration of different doses of Shao Gan Duo Gan.The effects of different treatments on serum ALT,AST and liver pathology were observed,and gene expression chips were used to observe changes in gene expression profile of the liver.Results Compared to model group,intragastric administration of high and intermediate doses of Shao Gan Duo Gan remarkably reduced the serum ALT in the injured rats [ （218.37±114.59,234.59±109.33） U/L vs （389.12±142.57） U/L,P〈0.05].The high dose of Shao Gan Duo Gan significantly decreased the histologic scores of the liver （2.42±0.52 vs 3.92±0.84,P〈0.05）,improved degeneration and necrosis of the hepatocytes,and upregulated or down-regulated expressions of genes related to liver jnjury.Conclusion Shao Gan Duo Ganhas an obvious liver protection,probably because it can regulate expressions of multiple genes related to the liver injury.It may be a drug which has an effect on multiple targets.

关 键 词：芍甘多苷 肝脏保护 基因表达谱 四氯化碳 模型 大鼠 

分 类 号：R285.5[医药卫生—中药学]