基于线粒体12S rRNA基因部分序列的6种浙南海域舌鳎属鱼类系统进化研究  

Phylogenetic Analysis of Six Species of Cynoglossus alone the South Coastal Area in Zhejiang Provinceon on Partial Sequence of Mitochondrial 12S Ribosomal RNA Gene

作　　者：於俊琦[1,2,3] 伍锦姑[2,3] 周志明[2,3] 曾国权[2,3] 单乐州[2,3] 刘伟成[2,3] 

机构地区：[1]温州医学院生命科学学院,浙江温州325000 [2]浙江省海洋水产养殖研究所,浙江温州325005 [3]浙江省近岸水域生物资源开发与保护重点实验室,浙江温州325005

出　　处：《科技通报》2012年第3期43-48,共6页Bulletin of Science and Technology

基　　金：浙江省科技厅重大科技攻关项目(2005C12006-02);浙江省科技厅创新团队建设与人才培养项目(2009F20009)

摘　　要：对浙江南部沿海的6种舌鳎属鱼类线粒体12S rRNA序列进行扩增,得到长度约为346 bp的片段。利用MEG Aversion3.0软件分析了6种鱼类的序列特征,检测到100个变异位点,其中简约信息位点43个,插入缺失位点11个;平均A+T∶C+G为57.2%∶43.8%。所有序列的转换颠换比(TS/TV)为2.8,突变未达到饱和。并以鳎科的塞内加尔鳎作为外群,利用PAUP Version4.0 b10软件构建了NJ、MP、ML系统进化树。结果显示在3种进化系统树中6种舌鳎的拓扑结构基本一致,大部分舌鳎间的系统进化关系与传统形态分类的结论吻合,但长吻红舌鳎的分类地位表现出较大分歧,研究认为以侧线和鳞片数量这两个形态特征作为评估舌鳎系统进化的重要性需要重新评估。This study sampled six Cynoglossus fishes in the southern coast of Zhejiang Province, and amplified the partial 12S rRNA gene sequences about 346bp. The sequences of these fish were analyzed using MEGAversion3.0 software, then we found 100 polymorphic sites including 43 parsimony-informative sites and 11 insertion/deletion and proportion of （A＋T）：（C＋G） is 57.2%：43.8%.The average ts/tv ratio among all pairwise comparisons was 2.8,suggesting that the se- quences had not reached saturation. Molecular phylogenetie trees were constructed by N J, MP and ML method using PAUP Version4.0 b 10 sofeware with outgroup Solea senegalensis. The results showed the topology of three trees are sim- ilar and consistent with the conclusions according to morphology excepting Cynoglossus lighti,it suggested that the importance of amount of lateral lines and scales in phylogenetic relationship need to reassess.

关 键 词：舌鳎 12S RRNA基因 系统发育 进化 

分 类 号：Q959.486[生物学—动物学]