准格尔露天矿区复垦对土壤细菌多样性的影响研究  

Influence of soil bacterial diversity in the process of reclamation in Jungar open coal mine of Inner Mongolia

作　　者：陈来红[1,2] 乔光华[1] 董红丽[3] 马万里[4] 

机构地区：[1]内蒙古农业大学经济管理学院,呼和浩特010019 [2]中国电力投资集团公司,北京100140 [3]内蒙古师范大学生命科学与技术学院,呼和浩特010022 [4]国家林业局林产工业规划设计院,北京100714

出　　处：《干旱区资源与环境》2012年第2期119-125,共7页Journal of Arid Land Resources and Environment

基　　金：国家自然科学基金项目(项目批准号:30760051 30560030);内蒙古自然科学基金项目(项目批准号:20080404MS0515);中国电力投资公司软课题(项目号:2009-189)资助

摘　　要：运用PCR-DGGE技术对我国准格尔露天煤矿不同复垦时间土壤细菌组成及其多样性变化进行了研究,结果表明:1)复垦区的土壤细菌主要是厚壁菌门、拟杆菌门、放线菌门、变形菌门、酸杆菌门、芽单胞菌门和未知细菌类7类,其中,放线菌门、拟杆菌门和变形菌门为优势类群,占到全部细菌的81.2%;2)成功完成矿山土壤样品微生物总DNA提取和PCR-DGGE的分析检测,在69个测序序列中,不可培养细菌占到总数的62.3%;3)各复垦阶段存在共有细菌类群,但更多的细菌类群是随复垦时间不同而发生变化的。4)豆科-禾本科牧草种植区的土壤细菌多样性指数要高于乔灌草类型,这可能与豆科植物中丰富的根瘤菌利于土壤细菌生长发育有关。To better understand the impact of the composition and the changes of the soil bacteria on processes of reclamation in Jungar open coal mine of Inner Mongolia, PCR - DGGE method was applied in this study success- fully. The phylogenetie affiliation of bacteria determined by 16S rDNA sequencing of cloned DNA fragments indi- cates that 69 soil bacterial sequences are mainly classified in 7 bacterial populations： Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria,Acidobacteria, Gemmatimonadetes and unclassified bacteria, among which Actinobac- teria, Bacteroidetes and Proteobacteria are the most dominant covering 81.2% of total soil bacteria. Meanwhile, 43 bacteria can not being cultured accounted for 62.3% ,were tested by PCR - DGGE. At different phases of in Jungar open coal mine, some bacteria species are changing, while there are certain inherent bacteria taxa at each reclamation phase. The soil bacteria diversity index the vegetation of Leguminosae and grasses is higher than that of trees, shrubs and herb, this may be relevant to rich leguminous plants.

关 键 词：准格尔露天矿区 复垦 土壤细菌群落 PCR-DGGE 

分 类 号：S154.381[农业科学—土壤学]